PMID- 16178442
OWN - NLM
STAT- MEDLINE
DCOM- 20060428
LR  - 20131121
IS  - 1000-503X (Print)
IS  - 1000-503X (Linking)
VI  - 27
IP  - 4
DP  - 2005 Aug
TI  - [Folate reduces monocyte chemoattractant protein-1 expression in rats with 
      hyperhomocysteinemia].
PG  - 470-4
AB  - OBJECTIVE: To investigate effects of supplementation of folate on the expression 
      of monocyte chemoattractant protein-1 (MCP-1) in aortic endothelium and release 
      from peripheral blood mononuclear cells (PBMC) in rats with hyperhomocysteinemia 
      induced by ingestion of excess methionine. METHODS: Thirty male SD rats were 
      randomly divided into 3 groups (n = 10 for each group): control group (Control), 
      high homocysteinemia group (Hhcy), and folate supplementation group (FA). They 
      were fed with nomal diet, normal diet enriched by 1.7% methionine, and normal 
      diet plus 1.7% methionine and 0.006% folate, respectively, for 45 days. The 
      levels of total plasma homocysteine (Thcy) were measured by high performance 
      liquid chromatography and the concentrations of chemokine MCP-1 released from 
      PBMC stimulated by oxidized low density lipoprotein were detected by enzyme 
      immunoassays. The expression of MCP-1 on aortas of rats was detected by 
      immunohistochemistry and Western blot. RESULTS: A high methionine diet for 45 
      days induced hyperhomocysteinemia. Folate supplementation to high-methionine diet 
      significantly decreased plasma Thcy levels (P < 0.01). The expression of MCP-1 in 
      aortic endothelium and the levels of MCP-1 released from PBMC stimulated by 
      oxidized low density lipoprotein were significantly higher in rats of Hhcy group 
      than in rats of control group (P < 0.05, P < 0.01). During supplementation of 
      folate, normalization of Thcy levels was accompanied by a marked reduction of 
      MCP-1 expression in aortic endothelium and by a significant decrease of MCP-1 
      released from PBMC stimulated by oxidized low density lipoprotein (P < 0.05, P < 
      0.01). CONCLUSION: Folate supplementation can prevent an elevation of 
      homocysteine levels in the blood and decrease the expression MCP-1 in aortic 
      endothelium and release of MCP-1 from PBMC in rats with hyperhomocysteinemia.
FAU - Chen, Jian
AU  - Chen J
AD  - Department of Cardiovasology, Union Hospital, Tongji Medical College, Huazhong 
      Science and Technology University, Wuhan 430022, China.
FAU - Li, Ming
AU  - Li M
FAU - Shu, Yan-Wen
AU  - Shu YW
FAU - Liao, Yu-Hua
AU  - Liao YH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao
JT  - Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
JID - 8006230
RN  - 0 (Chemokine CCL2)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 935E97BOY8 (Folic Acid)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Aorta/cytology/metabolism
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Endothelium, Vascular/metabolism
MH  - Folic Acid/*pharmacology
MH  - Homocysteine/blood
MH  - Hyperhomocysteinemia/blood/chemically induced/*metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Methionine
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/09/24 09:00
MHDA- 2006/04/29 09:00
CRDT- 2005/09/24 09:00
PHST- 2005/09/24 09:00 [pubmed]
PHST- 2006/04/29 09:00 [medline]
PHST- 2005/09/24 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Aug;27(4):470-4.