PMID- 16178658
OWN - NLM
STAT- MEDLINE
DCOM- 20051207
LR  - 20190930
IS  - 1083-3668 (Print)
IS  - 1083-3668 (Linking)
VI  - 10
IP  - 4
DP  - 2005 Jul-Aug
TI  - Direct visualization of mRNA colocalization with mitochondria in living cells 
      using molecular beacons.
PG  - 44025
AB  - The intracellular localization and specific organelle association of mRNA may 
      reflect essential functions, stages, and stability of mRNA. We report the direct 
      visualization of subcellular localization of K-ras and glyceraldehyde 3-phosphate 
      dehydrogenase (GAPDH) mRNAs in live HDF cells using molecular beacons together 
      with membrane-permeabilization and peptide-based delivery. Unexpectedly, we found 
      that both K-ras and GAPDH mRNAs colocalize with mitochondria. Extensive control 
      studies are performed, including the use of fluorescence in-situ hybridization 
      (FISH), negative-control beacons, and the detection of colocalization of 28S 
      ribosomal RNA with the rough endoplasmic reticulum (ER), suggesting that the mRNA 
      localization and colocalization patterns observed in our study are true and 
      specific. Our observation reveals intriguing subcellular associations of mRNA 
      with organelles such as mitochondria, which may provide new insight into the 
      transport, dynamics, and functions of mRNA and mRNA-protein interactions.
FAU - Santangelo, Philip J
AU  - Santangelo PJ
AD  - Georgia Institute of Technology and Emory University, Department of Biomedical 
      Engineering, Atlanta, Georgia 30332, USA.
FAU - Nitin, Nitin
AU  - Nitin N
FAU - Bao, Gang
AU  - Bao G
LA  - eng
GR  - 1 P20 GM072069-01/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biomed Opt
JT  - Journal of biomedical optics
JID - 9605853
RN  - 0 (RNA, Messenger)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
SB  - IM
MH  - Cell Line
MH  - Fibroblasts/*metabolism
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - Genes, ras/*genetics
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/*genetics/*metabolism
MH  - Humans
MH  - Microscopy, Fluorescence/methods
MH  - Mitochondria/*metabolism
MH  - *Molecular Probe Techniques
MH  - RNA, Messenger/*metabolism
EDAT- 2005/09/24 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/09/24 09:00
PHST- 2005/09/24 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/09/24 09:00 [entrez]
AID - 10.1117/1.2011402 [doi]
PST - ppublish
SO  - J Biomed Opt. 2005 Jul-Aug;10(4):44025. doi: 10.1117/1.2011402.