PMID- 16180097
OWN - NLM
STAT- MEDLINE
DCOM- 20060117
LR  - 20181113
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 278
IP  - 1-2
DP  - 2005 Oct
TI  - Lipopolysaccharide-induced alterations in oxygen consumption and radical 
      generation in endothelial cells.
PG  - 119-27
AB  - Oxygen consumption rate (OCR) and generation of superoxide and nitric oxide (NO) 
      in mouse aortic endothelial cells (MAECs) treated with lipopolysaccharide (LPS) 
      were studied. The OCR was determined in cell suspensions at 37 degrees C by 
      electron paramagnetic resonance (EPR) spectroscopy. LPS significantly altered the 
      OCR in a dose and time-dependent fashion. The OCR was significantly elevated 
      immediately following the treatment of MAECs with LPS (5 and 10 microg/ml) and 
      NADPH (100 microM) whereas the same was depressed 1 h after exposure to similar 
      conditions of incubation. Under similar experimental conditions, superoxide 
      generation was also determined by EPR spectroscopy and cytochrome c reduction 
      assays. A marginal increase in the superoxide production was observed when the 
      cells were treated with LPS and NADPH alone whereas the same was further enhanced 
      significantly when the cells were treated with LPS and NADPH together. The 
      increase in oxygen consumption and superoxide production caused by LPS was 
      inhibited by diphenyleneiodonium (DPI), suggesting the involvement of NAD(P)H 
      oxidase. A significant increase in the NO production by MAECs was noticed 1 h 
      after treatment with LPS and was inhibited by L-NAME, further suggesting the 
      involvement of nitric oxide synthase (NOS). Thus, on a temporal scale, 
      LPS-induced alterations in oxygen consumption by MAECs may be under the control 
      of dual regulation by NAD(P)H oxidase and NOS.
FAU - Pandian, Ramasamy P
AU  - Pandian RP
AD  - Center for Biomedical EPR Spectroscopy and Imaging, Davis Heart and Lung Research 
      Institute, Department of Internal Medicine, The Ohio State University, Columbus, 
      OH 43210, USA.
FAU - Kutala, Vijay Kumar
AU  - Kutala VK
FAU - Liaugminas, Alex
AU  - Liaugminas A
FAU - Parinandi, Narasimham L
AU  - Parinandi NL
FAU - Kuppusamy, Periannan
AU  - Kuppusamy P
LA  - eng
GR  - R01 EB004031/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Free Radicals)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Onium Compounds)
RN  - 11062-77-4 (Superoxides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 53-59-8 (NADP)
RN  - 6HJ411TU98 (diphenyleneiodonium)
SB  - IM
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Free Radicals/*metabolism
MH  - Lipopolysaccharides/metabolism/*pharmacology
MH  - NADP/metabolism
MH  - Nitric Oxide/metabolism
MH  - Onium Compounds/antagonists & inhibitors/metabolism
MH  - Oxygen Consumption/*drug effects/physiology
MH  - Superoxides/metabolism
MH  - Time Factors
EDAT- 2005/09/24 09:00
MHDA- 2006/01/18 09:00
CRDT- 2005/09/24 09:00
PHST- 2005/03/18 00:00 [received]
PHST- 2005/05/05 00:00 [accepted]
PHST- 2005/09/24 09:00 [pubmed]
PHST- 2006/01/18 09:00 [medline]
PHST- 2005/09/24 09:00 [entrez]
AID - 10.1007/s11010-005-6936-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2005 Oct;278(1-2):119-27. doi: 10.1007/s11010-005-6936-x.