PMID- 16180411
OWN - NLM
STAT- MEDLINE
DCOM- 20051108
LR  - 20220317
IS  - 0273-1223 (Print)
IS  - 0273-1223 (Linking)
VI  - 52
IP  - 1-2
DP  - 2005
TI  - Microbial characteristics of a methanogenic phenol-degrading sludge.
PG  - 73-8
AB  - Microbial properties of a methanogenic granular phenol-degrading sludge were 
      characterized using the 16S rRNA/DNA-based techniques, including polymerase chain 
      reaction (PCR) amplification, cloning, DNA sequencing, and fluorescence in situ 
      hybridization (FISH). The sludge was sampled from an upflow anaerobic sludge 
      blanket reactor, which removed 98% of phenol (up to 1260 mg/l) in wastewater at 
      26 degrees C with 12 hours of hydraulic retention. Based on DNA analysis, the 
      Eubacteria in the sludge was composed of 13 operational taxonomy units (OTUs). 
      Two OTUs, one resembling Clostridium and the other remotely resembling 
      Desulfotomaculum, were likely responsible for the conversion of phenol to 
      benzoate, which was further degraded by five Syntrophus-resembling OTUs to 
      acetate and H2/CO2; methanogens lastly converted acetate and H2/CO2 into methane. 
      The role of six remaining OTUs remains unclear. Overall, the sludge was composed 
      of 26 +/- 6% Eubacteria and 74 +/- 9% methanogens, of which 54 +/- 6% were 
      acetotrophic Methanosaetaceae, 14 +/- 3% and 3 +/- 2% were hydrogenotrophic 
      Methanomicrobiales and Methanobacteriaceae, respectively.
FAU - Zhang, T
AU  - Zhang T
AD  - Department of Civil Engineering, The University of Hong Kong, Pokfulam Road, Hong 
      Kong, China. hrechef@hkucc.hku.hk
FAU - Ke, S Z
AU  - Ke SZ
FAU - Liu, Y
AU  - Liu Y
FAU - Fang, H P
AU  - Fang HP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Water Sci Technol
JT  - Water science and technology : a journal of the International Association on 
      Water Pollution Research
JID - 9879497
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 339NCG44TV (Phenol)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria/classification/genetics/*isolation & purification/metabolism
MH  - Bacteria, Anaerobic/classification/genetics/*isolation & purification/metabolism
MH  - Base Sequence
MH  - Biodegradation, Environmental
MH  - Bioreactors
MH  - DNA, Archaeal/analysis
MH  - DNA, Bacterial/analysis
MH  - Euryarchaeota/classification/genetics/*isolation & purification/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/metabolism
MH  - Phenol/*metabolism
MH  - RNA, Ribosomal, 16S/analysis
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
EDAT- 2005/09/27 09:00
MHDA- 2005/11/09 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2005/11/09 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
PST - ppublish
SO  - Water Sci Technol. 2005;52(1-2):73-8.