PMID- 16180579
OWN - NLM
STAT- MEDLINE
DCOM- 20051212
LR  - 20131121
IS  - 1505-1773 (Print)
IS  - 1505-1773 (Linking)
VI  - 8
IP  - 3
DP  - 2005
TI  - Intracerebroventricular administration of PD 140.548 N-methyl-D-glucamine 
      attenuates the release of cortisol and catecholamines induced by duodenal 
      distension in the sheep.
PG  - 183-93
AB  - The aim of this study was to determine the influence of mechanically induced 
      duodenal distension (DD) and PD 140.548 N-methyl-D-glucamine (a specific peptide 
      antagonist of a CCK1 receptor) premedication on mechanographical reticulo-ruminal 
      activity, animal general behaviour, catecholamines (CA) and the blood plasma 
      cortisol levels, as well as the clinical symptoms of visceral pain induced by DD 
      in sheep. After 24 h fasting, 6 animals, Polish merino sheep were 
      praeanaesthetised by i.m. injection of ketamine (20 mg x kg(-1) b.w.) and 
      anaesthetised with i.v. infusion of pentobarbital (20 mg x kg(-1) b.w.) and a 
      permanent stainless steel cannula (gate cannula) was inserted inside the lateral 
      cerebral ventricle (controlled by cerebrospinal fluid efflux) 10 mm above the 
      bregma and 5 mm laterally from the midline suture using stereotaxic method. Under 
      the same general anaesthesia and analgesia a T-shaped silicon cannula, was 
      inserted into the duodenum (12 cm from pylorus) and a second one was inserted 
      into the dorsal sac of the rumen. During 7 consecutive days after surgery each 
      animal was treated i.m. with procaine penicillin (300000 I.U..kg(-1) b.w.), 
      dihydrostreptomycine (DHS, 10 microg x kg(-1) b.w.), prednisolone acetate 1.2 mg 
      x kg(-1) b.w.) together and i.m. injection of ketamine (20 mg x kg(-1) b.w.), 
      separetely. The influence of PD 140.548 N-methyl-D-glucamine on the unfavourable 
      effects of duodenal distension using a 10 cm long balloon filled with 40 and 80 
      ml (DD40 and DD80) water at animal body temperature was investigated in this 
      study. Five minutes DD40 and DD80 caused an immediate and compete inhibition of 
      the reticulo-ruminal frequency, a significant increase in plasma CA and cortisol 
      levels, an increase in the heart rate, hyperventilation and other symptoms of 
      pain, proportionally to the degree of intestinal distension. 
      Intracerebroventricular (i.c.v.) administration of PD 140.548 alone at a dose of 
      0.25, 0.5, 1 or 2 mg in toto did not significantly change the reticulo-ruminal 
      motility, CA and cortisol concentrations, but 10 min after the i.c.v. infusion 
      (or 10 min before DD) at a dose 1 and 2 mg in toto , it completely blocked the 
      increase of blood plasma cortisol, epinephrine (E), norepinephrine (NE) and 
      dopamine (DA) concentrations for 20 min. In the some time it prevented the 
      reticulo-ruminal atony provocked by DD. It is concluded that PD 140.548 
      N-methyl-D-glucamine--an antagonist of the central CCK1 receptor can be an 
      effective analgesic agent in duodenal pain. This action is due to the inhibition 
      of peripheral CCK1 type receptor in the central descending nerve pathway, 
      facilitating pain transmission in sheep perhaps in the 
      hypothalamic-pituitary-adrenal axis.
FAU - Kania, B F
AU  - Kania BF
AD  - Experimental and Clinical Physio-Pharmacological Laboratory, Department of 
      Physiological Sciences, Faculty of Veterinary Medicine, Agricultural University 
      of Warsaw, Poland. bfkania@alpha.sggw.waw.pl
FAU - Matczuk, J
AU  - Matczuk J
FAU - Kania, K
AU  - Kania K
FAU - Bueno, L
AU  - Bueno L
FAU - Fioramonti, J
AU  - Fioramonti J
FAU - Romanowicz, K
AU  - Romanowicz K
FAU - Sutiak, V
AU  - Sutiak V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pol J Vet Sci
JT  - Polish journal of veterinary sciences
JID - 101125473
RN  - 0 (Catecholamines)
RN  - 0 (Indoles)
RN  - 0 (PD 140.548 N-methyl-D-glucamine)
RN  - 0 (PD 140548)
RN  - 0 (Receptors, Cholecystokinin)
RN  - 6HG8UB2MUY (Meglumine)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Catecholamines/*blood
MH  - Dose-Response Relationship, Drug
MH  - Duodenum/*metabolism
MH  - Female
MH  - Gastrointestinal Motility/drug effects
MH  - Hydrocortisone/*blood
MH  - Indoles
MH  - Meglumine/*analogs & derivatives/pharmacology
MH  - Receptors, Cholecystokinin/*antagonists & inhibitors
MH  - Sheep/blood/*physiology
EDAT- 2005/09/27 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
PST - ppublish
SO  - Pol J Vet Sci. 2005;8(3):183-93.