PMID- 16181353
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20081121
IS  - 1083-4389 (Print)
IS  - 1083-4389 (Linking)
VI  - 10
IP  - 5
DP  - 2005 Oct
TI  - Helicobacter pylori outer membrane protein 18 (Hp1125) induces dendritic cell 
      maturation and function.
PG  - 424-32
AB  - BACKGROUND: Dendritic cells (DCs) are potent antigen-presenting cells that 
      initiate T-cell responses. A robust adaptive Th1 immune response is crucial to an 
      adaptive (Th2) immune response necessary for vaccine-induced protective immunity 
      against Helicobacter pylori. It has been shown that several outer membrane 
      proteins (Omps) induce a robust antibody response. However, it is also known that 
      the antibodies generated are not protective. Moreover there is great variation in 
      the recognition of high molecular weight H. pylori proteins by sera from infected 
      patients. In contrast to the high molecular weight proteins, serologic responses 
      to small molecular weight proteins provide assessment of current infection with 
      H. pylori and also of its eradication. AIM: The goal of the study was to analyze 
      the activation of the immune response by a specific low molecular weight Omp that 
      is universally expressed by all H. pylori strains. Therefore, we studied 
      interaction of H. pylori Omp18 with DCs. METHODS: Activation of murine bone 
      marrow-derived DCs and production of cytokines by Omp18 was assessed by 
      fluorescence-activated cell sorter (FACS) for costimulatory markers and ELISA, 
      respectively. The ability of Omp18 stimulated DCs to induce lymphocyte 
      proliferation was measured in a mixed leukocyte reaction. RESULTS: Omp18 induced 
      higher expression of the B7 (CD80 and CD86) costimulatory molecule after 18 hours 
      indicating processing and presentation of the antigen on the surface by bone 
      marrow-derived DCs. The maturing DCs also secreted significant levels of IL-12, 
      but was 4-fold less than that stimulated by whole bacteria. Omp18-primed DCs 
      induced proliferation and release of IFNgamma by syngeneic splenocytes. 
      CONCLUSION: We concluded that Omp18 is capable of activating DCs initiating a Th1 
      immune response.
FAU - Rathinavelu, Sivaprakash
AU  - Rathinavelu S
AD  - Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, 
      USA.
FAU - Kao, John Y
AU  - Kao JY
FAU - Zavros, Yana
AU  - Zavros Y
FAU - Merchant, Juanita L
AU  - Merchant JL
LA  - eng
GR  - P01 DK 61410/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Helicobacter
JT  - Helicobacter
JID - 9605411
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Bacterial Outer Membrane Proteins/genetics/*immunology
MH  - Cell Differentiation
MH  - Dendritic Cells/*cytology/*immunology
MH  - Female
MH  - Helicobacter Infections/immunology/microbiology
MH  - Helicobacter pylori/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Recombinant Proteins/immunology
MH  - Th1 Cells/immunology
EDAT- 2005/09/27 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
AID - HEL350 [pii]
AID - 10.1111/j.1523-5378.2005.00350.x [doi]
PST - ppublish
SO  - Helicobacter. 2005 Oct;10(5):424-32. doi: 10.1111/j.1523-5378.2005.00350.x.