PMID- 16181710
OWN - NLM
STAT- MEDLINE
DCOM- 20061121
LR  - 20060601
IS  - 0264-410X (Print)
IS  - 0264-410X (Linking)
VI  - 24
IP  - 21
DP  - 2006 May 22
TI  - Immunological cross-reactivity against a drug mutated HIV-1 protease epitope 
      after DNA multi-CTL epitope construct immunization.
PG  - 4527-30
AB  - Epitopes in HIV polymerase were analyzed by peptide binding to human leukocyte 
      antigen (HLA) A0201 molecules, the most frequent HLA class in the Caucasian 
      population. We found that HIV-1 protease peptides representing both the wild type 
      and anticipated drug resistance variants of the sequence bound well to HLA-A0201. 
      We also found that wild type as well as a double mutated variant of the epitope 
      was strongly immunogenic in HLA-A0201 transgenic mice, either as individual 
      peptides or encoded in DNA multi-CTL epitope constructs. Immunological 
      cross-reactivity between different variants of the peptide could be seen, 
      suggesting that it may be possible to induce a broad immune response by 
      immunizing with drug resistance-mutated epitopes. This may be of advantage for 
      HIV-1 infected patients since such a response may cause a better outcome of an 
      anti-retroviral drug therapy.
FAU - Boberg, Andreas
AU  - Boberg A
AD  - Swedish Institute for Infectious Disease Control, Karolinska Institute, 171 82 
      Solna, Sweden. andreas.boberg@smi.ki.se
FAU - Sjostrand, Dan
AU  - Sjostrand D
FAU - Rollman, Erik
AU  - Rollman E
FAU - Hinkula, Jorma
AU  - Hinkula J
FAU - Zuber, Bartek
AU  - Zuber B
FAU - Wahren, Britta
AU  - Wahren B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050818
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Epitopes)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (HLA Antigens)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.23.- (HIV Protease)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - *Cross Reactions
MH  - Epitopes/*immunology
MH  - Female
MH  - HIV Envelope Protein gp120/genetics/immunology
MH  - HIV Protease/genetics/*immunology
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - *Mutation
MH  - RNA, Messenger/genetics
MH  - Sequence Homology, Amino Acid
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 2005/09/27 09:00
MHDA- 2006/12/09 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
AID - S0264-410X(05)00815-7 [pii]
AID - 10.1016/j.vaccine.2005.08.019 [doi]
PST - ppublish
SO  - Vaccine. 2006 May 22;24(21):4527-30. doi: 10.1016/j.vaccine.2005.08.019. Epub 
      2005 Aug 18.