PMID- 16182303
OWN - NLM
STAT- MEDLINE
DCOM- 20061129
LR  - 20091111
IS  - 0021-9797 (Print)
IS  - 0021-9797 (Linking)
VI  - 296
IP  - 1
DP  - 2006 Apr 1
TI  - Electroactive films of heme protein-coated multiwalled carbon nanotubes.
PG  - 204-11
AB  - A novel method for fabricating protein-MWNT films on pyrolytic graphite (PG) 
      electrodes was described. Positively charged hemoglobin (Hb) or myoglobin (Mb) in 
      buffers at pH 5.5 or 5.0 was first adsorbed on the surface of acid-pretreated, 
      negatively charged multiwalled carbon nanotubes (MWNTs) mainly by electrostatic 
      interaction, forming a core-shell structure. The aqueous dispersion of 
      protein-coated MWNTs was then cast on PG electrodes, forming protein-MWNT films 
      after evaporation of solvent. The protein-MWNT films exhibited a pair of 
      well-defined, quasi-reversible cyclic voltammetric peaks, characteristic of heme 
      Fe(III)/Fe(II) redox couples. The protein films were characterized by 
      voltammetry, UV-vis spectroscopy, and scanning electron microscopy (SEM). This 
      approach for assembly of protein-MWNT films showed higher surface concentration 
      of electroactive proteins than the simple cast method, and the amount of proteins 
      in the films could be controlled more precisely compared with the dipping method. 
      Furthermore, the film assembly using this method was more stable than that using 
      simple cast method. The proteins in MWNT films retained their near-native 
      structure, and electrochemically catalyzed reduction of oxygen and hydrogen 
      peroxide, suggesting the potential applicability of the films as the new type of 
      biosensors or bioreactors based on direct electrochemistry of enzymes.
FAU - Zhao, Liyun
AU  - Zhao L
AD  - Department of Chemistry, Beijing Normal University, Beijing 100875, China.
FAU - Liu, Hongyun
AU  - Liu H
FAU - Hu, Naifei
AU  - Hu N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050922
PL  - United States
TA  - J Colloid Interface Sci
JT  - Journal of colloid and interface science
JID - 0043125
RN  - 0 (Hemoglobins)
RN  - 0 (Myoglobin)
RN  - 0 (Nanotubes, Carbon)
SB  - IM
MH  - Electrochemistry
MH  - Hemoglobins/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Electron, Transmission
MH  - Molecular Conformation
MH  - Myoglobin/*chemistry
MH  - Nanotubes, Carbon/*chemistry
MH  - Surface Properties
EDAT- 2005/09/27 09:00
MHDA- 2006/12/09 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/07/12 00:00 [received]
PHST- 2005/08/22 00:00 [revised]
PHST- 2005/08/22 00:00 [accepted]
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2006/12/09 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
AID - S0021-9797(05)00893-3 [pii]
AID - 10.1016/j.jcis.2005.08.041 [doi]
PST - ppublish
SO  - J Colloid Interface Sci. 2006 Apr 1;296(1):204-11. doi: 
      10.1016/j.jcis.2005.08.041. Epub 2005 Sep 22.