PMID- 16184387
OWN - NLM
STAT- MEDLINE
DCOM- 20060804
LR  - 20181113
IS  - 0175-7571 (Print)
IS  - 0175-7571 (Linking)
VI  - 35
IP  - 1
DP  - 2005 Dec
TI  - Dynamics of a membrane-bound tryptophan analog in environments of varying 
      hydration: a fluorescence approach.
PG  - 62-71
AB  - Tryptophan octyl ester (TOE) represents an important model for membrane-bound 
      tryptophan residues. In this article, we have employed a combination of 
      wavelength-selective fluorescence and time-resolved fluorescence spectroscopies 
      to monitor the effect of varying degrees of hydration on the dynamics of TOE in 
      reverse micellar environments formed by sodium bis(2-ethylhexyl) sulfosuccinate 
      (AOT) in isooctane. Our results show that TOE exhibits red edge excitation shift 
      (REES) and other wavelength-selective fluorescence effects when bound to reverse 
      micelles of AOT. Fluorescence parameters such as intensity, emission maximum, 
      anisotropy, and lifetime of TOE in reverse micelles of AOT depend on 
      [water]/[surfactant] molar ratio (w (o)). These results are relevant and 
      potentially useful for analyzing dynamics of proteins or peptides bound to 
      membranes or membrane-mimetic media under conditions of changing hydration.
FAU - Chattopadhyay, Amitabha
AU  - Chattopadhyay A
AD  - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India. 
      amit@ccmb.res.in
FAU - Arora, Ajuna
AU  - Arora A
FAU - Kelkar, Devaki A
AU  - Kelkar DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050924
PL  - Germany
TA  - Eur Biophys J
JT  - European biophysics journal : EBJ
JID - 8409413
RN  - 0 (Micelles)
RN  - 0 (Octanes)
RN  - 0 (Surface-Active Agents)
RN  - 0 (tryptophan octyl ester)
RN  - 059QF0KO0R (Water)
RN  - 10041-19-7 (Dioctyl Sulfosuccinic Acid)
RN  - 8DUH1N11BX (Tryptophan)
RN  - QAB8F5669O (2,2,4-trimethylpentane)
SB  - IM
MH  - Biomimetics
MH  - Dioctyl Sulfosuccinic Acid/chemistry
MH  - Fluorescence
MH  - Membranes/*chemistry
MH  - Micelles
MH  - Octanes/chemistry
MH  - Spectrometry, Fluorescence
MH  - Surface-Active Agents/chemistry
MH  - Tryptophan/*analogs & derivatives/chemistry
MH  - Water/*chemistry
EDAT- 2005/09/27 09:00
MHDA- 2006/08/05 09:00
CRDT- 2005/09/27 09:00
PHST- 2005/03/15 00:00 [received]
PHST- 2005/07/18 00:00 [accepted]
PHST- 2005/06/30 00:00 [revised]
PHST- 2005/09/27 09:00 [pubmed]
PHST- 2006/08/05 09:00 [medline]
PHST- 2005/09/27 09:00 [entrez]
AID - 10.1007/s00249-005-0009-7 [doi]
PST - ppublish
SO  - Eur Biophys J. 2005 Dec;35(1):62-71. doi: 10.1007/s00249-005-0009-7. Epub 2005 
      Sep 24.