PMID- 16187758
OWN - NLM
STAT- MEDLINE
DCOM- 20051110
LR  - 20190917
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 164
IP  - 4 Pt 2
DP  - 2005 Oct
TI  - Caffeine sensitizes nondividing human fibroblasts to x rays by inducing a high 
      frequency of misrepair.
PG  - 509-13
AB  - Caffeine sensitizes cells to ionizing radiation, and this effect is believed to 
      be associated with the disruption of DNA damage-responsive cell cycle 
      checkpoints, which is controlled by ATM. Recent studies suggest that misrejoining 
      of DSBs is one of the underlying mechanisms of AT cell hyper-radiosensitivity. In 
      this study, we investigated the effects of caffeine and radiation on nongrowing 
      G0 normal human fibroblast cells by determining cell survival and scoring 
      aberrations in calyculin A-induced G2 chromosomes. Results from the cell survival 
      study indicate that after X-ray exposure G0 cells were sensitized by 24 h 
      treatment with caffeine. Analysis of chromosome aberrations using FISH 
      (fluorescence in situ hybridization) revealed a high frequency of aberrant cells 
      and color junctions in the caffeine-treated cells. Since most DNA repair in 
      nongrowing G0 cells is believed to result from nonhomologous end joining (NHEJ), 
      caffeine may influence the fidelity of the NHEJ pathway in irradiated G0 cells.
FAU - Kawata, Tetsuya
AU  - Kawata T
AD  - Department of Radiology, Graduate School of Medicine, Chiba, Japan. 
      tkawata@faculty.chiba-u.jp
FAU - Ito, Hisao
AU  - Ito H
FAU - Saito, Masayoshi
AU  - Saito M
FAU - Uno, Takashi
AU  - Uno T
FAU - Okayasu, Ryuichi
AU  - Okayasu R
FAU - Liu, Cuihua
AU  - Liu C
FAU - Kan'o, Momoe
AU  - Kan'o M
FAU - George, Kerry
AU  - George K
FAU - Cucinotta, Francis A
AU  - Cucinotta FA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Radiation-Sensitizing Agents)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Caffeine/*pharmacology
MH  - Cell Line
MH  - Chromosome Aberrations
MH  - DNA Repair/*drug effects
MH  - Fibroblasts/drug effects/*radiation effects
MH  - Humans
MH  - Radiation-Sensitizing Agents/*pharmacology
MH  - Recombination, Genetic/drug effects
MH  - X-Rays
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Cucinotta, F A
IR  - Cucinotta FA
IRAD- JSC
EDAT- 2005/09/29 09:00
MHDA- 2005/11/11 09:00
CRDT- 2005/09/29 09:00
PHST- 2005/09/29 09:00 [pubmed]
PHST- 2005/11/11 09:00 [medline]
PHST- 2005/09/29 09:00 [entrez]
AID - RR3382 [pii]
AID - 10.1667/rr3382.1 [doi]
PST - ppublish
SO  - Radiat Res. 2005 Oct;164(4 Pt 2):509-13. doi: 10.1667/rr3382.1.