PMID- 16189006
OWN - NLM
STAT- MEDLINE
DCOM- 20051108
LR  - 20240412
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 20
DP  - 2005 Oct
TI  - Human herpesvirus 6 open reading frame U14 protein and cellular p53 interact with 
      each other and are contained in the virion.
PG  - 13037-46
AB  - A mass spectroscopic analysis of proteins from human herpesvirus 6 
      (HHV-6)-infected cells showed that the HHV-6 U14 protein coimmunoprecipitated 
      with the tumor suppressor p53. The binding of U14 to p53 was verified by 
      coimmunoprecipitation experiments in both Molt-3 cells infected with HHV-6 and 
      293 cells cotransfected with U14 and p53 expression vectors. Indirect 
      immunofluorescence assays (IFAs) showed that by 18 h postinfection (hpi) U14 
      localized to the dot-like structures observed in both the nucleus and cytoplasm 
      where p53 was partly accumulated. Despite Northern blotting evidence that U14 
      follows late kinetics, the U14 protein was detected immediately after infection 
      (at 3 hpi) by IFA. In addition, by Western blotting, U14 was detected at 0 hpi or 
      in the presence of cycloheximide which completely abolished the expression of IE1 
      protein. In addition to U14, p53 was detected at 0 hpi although it was not 
      detected in mock-infected cells. Furthermore, both U14 and p53 were clearly 
      detected in the viral particles by Western blotting and immunoelectron 
      microscopy, supporting the idea that U14 and p53 are incorporated into virions. 
      Our study provides the first evidence of the incorporation of cellular p53 into 
      viral particles and suggests that p53 may play an important role in viral 
      infection.
FAU - Takemoto, Masaya
AU  - Takemoto M
AD  - Department of Microbiology, Osaka University Graduate School of Medicine C1, 
      Japan.
FAU - Koike, Masato
AU  - Koike M
FAU - Mori, Yasuko
AU  - Mori Y
FAU - Yonemoto, Sayoko
AU  - Yonemoto S
FAU - Sasamoto, Yumi
AU  - Sasamoto Y
FAU - Kondo, Kazuhiro
AU  - Kondo K
FAU - Uchiyama, Yasuo
AU  - Uchiyama Y
FAU - Yamanishi, Koichi
AU  - Yamanishi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Viral Structural Proteins)
SB  - IM
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - Herpesvirus 6, Human/chemistry/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Kinetics
MH  - Microscopy, Immunoelectron
MH  - Molecular Weight
MH  - Open Reading Frames
MH  - Protein Binding
MH  - Tumor Suppressor Protein p53/*metabolism
MH  - Viral Structural Proteins/*metabolism
MH  - Virion/chemistry/*metabolism
PMC - PMC1235810
EDAT- 2005/09/29 09:00
MHDA- 2005/11/09 09:00
PMCR- 2005/10/01
CRDT- 2005/09/29 09:00
PHST- 2005/09/29 09:00 [pubmed]
PHST- 2005/11/09 09:00 [medline]
PHST- 2005/09/29 09:00 [entrez]
PHST- 2005/10/01 00:00 [pmc-release]
AID - 79/20/13037 [pii]
AID - 0032-05 [pii]
AID - 10.1128/JVI.79.20.13037-13046.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 Oct;79(20):13037-46. doi: 10.1128/JVI.79.20.13037-13046.2005.