PMID- 16189275
OWN - NLM
STAT- MEDLINE
DCOM- 20060207
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 107
IP  - 2
DP  - 2006 Jan 15
TI  - Role of dendritic cell-derived CXCL13 in the pathogenesis of Bartonella henselae 
      B-rich granuloma.
PG  - 454-62
AB  - Dendritic cells (DCs) initiate adaptive immunity and regulate the inflammatory 
      response by producing inflammatory chemokines. This study was aimed to elucidate 
      their role in the pathogenesis of the suppurative granuloma induced by Bartonella 
      henselae infection, which characterizes cat scratch disease (CSD). In vitro DC 
      infection by B. henselae results in internalization of bacteria, phenotypic 
      maturation with increased expression of HLA-DR and CD86, and induction of CD83, 
      CD208, and CCR7. In comparison to LPS-activated DCs, B henselae-infected DCs 
      produce higher amounts of IL-10, whereas the production of IL-12p70 is reduced. 
      Infected DCs also produce high levels of CXCL8 and CXCL13, 2 chemokines active 
      respectively on neutrophils and B lymphocytes. These results provide the 
      molecular basis for the morphogenesis of CSD granuloma, which typically contains 
      high numbers of neutrophils and B cells. Remarkably, CSD granulomas in vivo 
      contain CXCL13-producing DCs. We further demonstrate that the B cells in CSD 
      granulomas are represented by monocytoid B cells and, worth noting, they express 
      T-bet, a transcription factor able to induce a T-independent immunoglobulin (Ig) 
      class switch in B lymphocytes. These findings suggest that the humoral immune 
      response to B henselae initiates in the extrafollicular areas of infected lymph 
      nodes and is regulated by DCs.
FAU - Vermi, William
AU  - Vermi W
AD  - Department of Pathology and the Section of General Pathology and Immunology, 
      University of Brescia, Italy.
FAU - Facchetti, Fabio
AU  - Facchetti F
FAU - Riboldi, Elena
AU  - Riboldi E
FAU - Heine, Holger
AU  - Heine H
FAU - Scutera, Sara
AU  - Scutera S
FAU - Stornello, Sarah
AU  - Stornello S
FAU - Ravarino, Daniela
AU  - Ravarino D
FAU - Cappello, Paola
AU  - Cappello P
FAU - Giovarelli, Mirella
AU  - Giovarelli M
FAU - Badolato, Raffaele
AU  - Badolato R
FAU - Zucca, Mario
AU  - Zucca M
FAU - Gentili, Francesca
AU  - Gentili F
FAU - Chilosi, Marco
AU  - Chilosi M
FAU - Doglioni, Claudio
AU  - Doglioni C
FAU - Ponzi, Alessandro Negro
AU  - Ponzi AN
FAU - Sozzani, Silvano
AU  - Sozzani S
FAU - Musso, Tiziana
AU  - Musso T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050927
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (CXCL13 protein, human)
RN  - 0 (Chemokine CXCL13)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Transcription Factors)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/immunology/metabolism
MH  - Bartonella henselae/isolation & purification
MH  - Cat-Scratch Disease/immunology/microbiology/*pathology
MH  - Cats
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CXCL13
MH  - Chemokines, CXC/*metabolism
MH  - Dendritic Cells/*metabolism
MH  - Granuloma/immunology/microbiology/*pathology
MH  - Humans
MH  - Immunoglobulin G/metabolism
MH  - Interleukin-10/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lymph Nodes/*pathology
MH  - T-Box Domain Proteins
MH  - T-Lymphocytes/immunology/metabolism
MH  - Toll-Like Receptors/immunology/metabolism
MH  - Transcription Factors/metabolism
EDAT- 2005/09/29 09:00
MHDA- 2006/02/08 09:00
CRDT- 2005/09/29 09:00
PHST- 2005/09/29 09:00 [pubmed]
PHST- 2006/02/08 09:00 [medline]
PHST- 2005/09/29 09:00 [entrez]
AID - S0006-4971(20)67277-9 [pii]
AID - 10.1182/blood-2005-04-1342 [doi]
PST - ppublish
SO  - Blood. 2006 Jan 15;107(2):454-62. doi: 10.1182/blood-2005-04-1342. Epub 2005 Sep 
      27.