PMID- 16189666
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20181113
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 57
IP  - 10
DP  - 2005 Nov
TI  - Extended HLA-DPB1 polymorphism: an RNA approach for HLA-DPB1 typing.
PG  - 790-4
AB  - Most of the 119 human leukocyte antigen (HLA)-DPB1 alleles are defined by 
      polymorphism in six hypervariable regions (HVRs) in exon 2 of the HLA-DPB1 gene. 
      We investigated how DPB1 polymorphism is represented in the entire coding region. 
      An RNA sequencing-based typing (SBT) approach was developed for the 
      identification of HLA-DPB1 polymorphism from the 5' untranslated region (UTR) 
      through the 3'-UTR. B-cell lymphoblastoid cell lines, encoding 16 different DPB1 
      alleles, were studied. Results show additional HLA-DPB1 polymorphism in exons 1, 
      3, 4 and 5 and the 5' and 3'-UTR. Four new HLA-DPB1 alleles were identified, 
      DPB1*0502, DPB1*0602, DPB1*0802 and DPB1*0902, which have exon 2 sequences 
      identical to other DPB1 alleles but differ in the extended region. The additional 
      polymorphism represents two main polymorphic lineages in the DPB1 alleles. Among 
      the HVRs in exon 2, only HVR F correlates with these two main lineages.
FAU - Reinders, Judith
AU  - Reinders J
AD  - Department of Pathology, H04.312, University Medical Centre Utrecht (UMC-U), 
      Heidelberglaan 100, PO Box 85500, 3508 GA, Utrecht, The Netherlands.
FAU - Rozemuller, Erik H
AU  - Rozemuller EH
FAU - van Gent, Rogier
AU  - van Gent R
FAU - Arts-Hilkes, Yvonne H A
AU  - Arts-Hilkes YH
FAU - van den Tweel, Jan G
AU  - van den Tweel JG
FAU - Tilanus, Marcel G J
AU  - Tilanus MG
LA  - eng
PT  - Journal Article
DEP - 20051108
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (HLA-DP Antigens)
RN  - 0 (HLA-DP beta-Chains)
RN  - 0 (HLA-DPB1 antigen)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Exons
MH  - HLA-DP Antigens/*genetics/immunology
MH  - HLA-DP beta-Chains
MH  - Humans
MH  - *Polymorphism, Genetic
MH  - RNA/*genetics
EDAT- 2005/09/29 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/09/29 09:00
PHST- 2005/05/17 00:00 [received]
PHST- 2005/08/10 00:00 [accepted]
PHST- 2005/09/29 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/09/29 09:00 [entrez]
AID - 10.1007/s00251-005-0043-8 [doi]
PST - ppublish
SO  - Immunogenetics. 2005 Nov;57(10):790-4. doi: 10.1007/s00251-005-0043-8. Epub 2005 
      Nov 8.