PMID- 16192698
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20061115
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 111
IP  - 3-4
DP  - 2005
TI  - Individual variation in the frequency of sperm aneuploidy in humans.
PG  - 229-36
AB  - To examine interindividual differences in sperm chromosome aneuploidy, repeated 
      semen specimens were obtained from a group of ten healthy men, aged 20-21 at the 
      start of the study, and analyzed by multi-color fluorescence in situ 
      hybridization (FISH) analysis to determine the frequencies of sperm aneuploidy 
      for chromosomes X, Y, 8, 18 and 21 and of diploidy. Semen samples were obtained 
      three times over a five-year period. Statistical analysis examining the stability 
      of sperm aneuploidy over time by type and chromosome identified two men who 
      consistently exhibited elevated frequencies of sperm aneuploidy (stable 
      variants): one with elevated disomy 18 and one with elevated MII diploidy. 
      Differences among frequencies of aneuploidy by chromosome were also seen. 
      Overall, disomy frequencies were lower for chromosome X, 8 and 18 than for 
      chromosomes 21 or Y and for XY aneuploidy. The frequency of chromosome Y disomy 
      did not differ from XY sperm frequency. Also, the frequency of meiosis I (XY) and 
      II (YY + XX) sex chromosome errors did not differ in haploid sperm, but the 
      frequency of MII errors was lower than MI errors in diploid sperm. Frequencies of 
      sperm aneuploidy were similar between the first sampling period and the second, 
      two years later. However, the frequency of some types of aneuploidy (XY, disomy 
      Y, disomy 8, total autosomal disomies, total diploidy, and subcategories of 
      diploidy) increased significantly between the first sampling period and the last, 
      five years later, while others remained unchanged (disomy X, 21 and 18). These 
      findings confirm inter-chromosome differences in the frequencies of disomy and 
      suggest that some apparently healthy men exhibit consistently elevated 
      frequencies of specific sperm aneuplodies. Furthermore, time/age-related changes 
      in sperm aneuploidy may be detected over as short a period as five years in a 
      repeated-measures study.
CI  - Copyright 2005 S. Karger AG, Basel.
FAU - Rubes, J
AU  - Rubes J
AD  - Veterinary Research Institute, Brno, Czech Republic. rubes@vri.cz
FAU - Vozdova, M
AU  - Vozdova M
FAU - Oracova, E
AU  - Oracova E
FAU - Perreault, S D
AU  - Perreault SD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Semen/cytology/physiology
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/cytology/*physiology
MH  - Uniparental Disomy/genetics
EDAT- 2005/09/30 09:00
MHDA- 2006/01/24 09:00
CRDT- 2005/09/30 09:00
PHST- 2004/10/29 00:00 [received]
PHST- 2005/01/20 00:00 [accepted]
PHST- 2005/09/30 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/09/30 09:00 [entrez]
AID - 86893 [pii]
AID - 10.1159/000086893 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2005;111(3-4):229-36. doi: 10.1159/000086893.