PMID- 16193165
OWN - NLM
STAT- MEDLINE
DCOM- 20060105
LR  - 20131121
IS  - 1464-0325 (Print)
IS  - 1464-0325 (Linking)
VI  - 7
IP  - 10
DP  - 2005 Oct
TI  - Bioaerosol characterization by flow cytometry with fluorochrome.
PG  - 950-9
AB  - Traditional culture and microscopy methods for evaluation of bioaerosols are 
      slow, tedious, and rather imprecise. In this study, the application of flow 
      cytometry that was combined with a fluorescent technique (FCM/FL) was evaluated 
      as a technique to quickly and accurately determine and quantify the total 
      concentration and viability of bioaerosols. The optimal conditions of five 
      fluorescent dyes [acridine orange (AO), SYTO-13, propidium iodide (PI), YOPRO-1, 
      and 5-cyano-2,3-ditolytetrazolium chloride (CTC)] used in FCM/FL were determined 
      for laboratory samples of bacterial aerosols (Escherichia coli, and endospores of 
      Bacillus subtilis) and fungal aerosols (Candida famata and Penicillium citrinum 
      spores). Based on the measured cell concentration, fluorescence intensity, and 
      staining efficiency as indicators for dye performance evaluation, SYTO-13 was 
      found to be the most suitable fluorescent dye for determining the total 
      concentration of the bioaerosols, as well as YOPRO-1 was the most suitable for 
      determining viability. Moreover, the established optimal FCM/FL with dyes was 
      validated for characterizing microorganism profiles from both air and water 
      samples from the aeration tank of hospital wastewater treatment plant. In 
      conclusion, the FCM/FL successfully assessed the total concentration and 
      viability for bacterial and fungal microorganisms in environmental field samples.
FAU - Chen, Pei-Shih
AU  - Chen PS
AD  - Graduate Institute of Environmental Health, College of Public Health, National 
      Taiwan University, Taipei, 100, Taiwan, ROC.
FAU - Li, Chih-Shan
AU  - Li CS
LA  - eng
PT  - Journal Article
DEP - 20050812
PL  - England
TA  - J Environ Monit
JT  - Journal of environmental monitoring : JEM
JID - 100968688
RN  - 0 (Aerosols)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Organic Chemicals)
RN  - 0 (SYTO 13)
RN  - 0 (Tetrazolium Salts)
RN  - 102568-47-8 (5-cyano-2,3-ditolyltetrazolium)
RN  - 36015-30-2 (Propidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry
MH  - Aerosols/*analysis
MH  - *Air Microbiology
MH  - Bacteria/*isolation & purification
MH  - Flow Cytometry/*methods
MH  - Fluorescent Dyes/*chemistry
MH  - Fungi/*isolation & purification
MH  - Organic Chemicals/chemistry
MH  - Propidium/chemistry
MH  - Tetrazolium Salts/chemistry
MH  - Water Microbiology
EDAT- 2005/09/30 09:00
MHDA- 2006/01/06 09:00
CRDT- 2005/09/30 09:00
PHST- 2005/09/30 09:00 [pubmed]
PHST- 2006/01/06 09:00 [medline]
PHST- 2005/09/30 09:00 [entrez]
AID - 10.1039/b505224f [doi]
PST - ppublish
SO  - J Environ Monit. 2005 Oct;7(10):950-9. doi: 10.1039/b505224f. Epub 2005 Aug 12.