PMID- 16193457
OWN - NLM
STAT- MEDLINE
DCOM- 20060106
LR  - 20051005
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 25
IP  - 10
DP  - 2005 Oct
TI  - Polar body biopsy and aneuploidy testing by simultaneous detection of six 
      chromosomes.
PG  - 867-71
AB  - OBJECTIVES: To simultaneously detect six chromosomes in a single round of 
      fluorescence in situ hybridization (FISH) during polar body diagnosis and 
      aneuploidy testing in human in vitro fertilization (IVF) treatment. METHODS: A 
      commercially available five-color FISH probe was modified by an additional 
      chromosome probe. This kit was first tested on lymphocyte spreads and then used 
      for polar body diagnosis (PBD) in patients with advanced maternal age and 
      repeated implantation failure. The outcome of IVF treatment was compared with a 
      control group. RESULTS: All six chromosomes could be simultaneously detected and 
      easily distinguished by FISH analysis. PBD and aneuploidy testing were performed 
      in 75 treatment cycles and compared with 126 controls. The biochemical pregnancy 
      rate was significantly higher in the PBD group (37.1% vs 22.9%, p < 0.05) and a 
      trend was observed for higher clinical pregnancy and implantation rates (24.22% 
      and 14.4% vs 18.62% and 10.8%, respectively) and lower abortion rates (20% vs 
      31.8%) following PBD. CONCLUSIONS: The simultaneous detection of six chromosomes 
      in a single FISH round is possible and can be applied to PBD. This approach may 
      present another step towards increasing the number of chromosomes for aneuploidy 
      testing.
CI  - Copyright 2005 John Wiley & Sons, Ltd.
FAU - Montag, Markus
AU  - Montag M
AD  - Department of Gynecological Endocrinology and Reproductive Medicine, University 
      Clinics of Bonn, Germany. markus.montag@uni-bonn.de
FAU - Limbach, Nina
AU  - Limbach N
FAU - Sabarstinski, Mareike
AU  - Sabarstinski M
FAU - van der Ven, Katrin
AU  - van der Ven K
FAU - Dorn, Christoph
AU  - Dorn C
FAU - van der Ven, Hans
AU  - van der Ven H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Biopsy
MH  - Chromosomes, Human/*ultrastructure
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Maternal Age
MH  - Oocytes/*ultrastructure
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
EDAT- 2005/09/30 09:00
MHDA- 2006/01/07 09:00
CRDT- 2005/09/30 09:00
PHST- 2005/09/30 09:00 [pubmed]
PHST- 2006/01/07 09:00 [medline]
PHST- 2005/09/30 09:00 [entrez]
AID - 10.1002/pd.1156 [doi]
PST - ppublish
SO  - Prenat Diagn. 2005 Oct;25(10):867-71. doi: 10.1002/pd.1156.