PMID- 16193516
OWN - NLM
STAT- MEDLINE
DCOM- 20060418
LR  - 20081121
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 93
IP  - 2
DP  - 2006 Feb 5
TI  - A novel microsurgery method for intact plant tissue at the single cell level 
      using ArF excimer laser microprojection.
PG  - 325-31
AB  - A novel microsurgery technique for the partial removal of rigid cell-walls in 
      intact plant tissue is established. Using a size-variable slit, an ArF excimer 
      laser was microprojected on the surface of the targeted cell, and this method 
      enabled the area- and depth-controllable processing of the cortical structure of 
      plant cells including the cuticle and cell wall layer. In epidermal cells of all 
      tested plants, viabilities of more than 90% were retained 24 h after irradiation. 
      Scanning electron microscope (SEM) observation revealed that the cuticle layer of 
      the irradiated region was completely ablated, and the cellulose microfibrils of 
      the secondary cell wall were partially removed; furthermore, 4 days after laser 
      treatment, the regeneration of cell wall fibrils was observed. As a model 
      experiment, the transient expression of synthetic green fluorescent protein 
      (sGFP) was performed by the microinjection of cauliflower mosaic virus (CMV) 35S 
      promoter-derived sGFP gene through an "aperture" in the treated cell surface. 
      Moreover, micron-sized fluorescent beads were successfully introduced by the same 
      method into the onion cells indicating that this method can be used to introduce 
      foreign materials as large as organelles.
CI  - (c) 2005 Wiley Periodicals, Inc.
FAU - Kajiyama, Shin'ichiro
AU  - Kajiyama S
AD  - Department of Biotechnology Graduate School of Engineering, Osaka University 2-1 
      Yamadaoka, Suita, Osaka 565-0871, Japan. kaiyama@bio.eng.osaka-u.ac.jp
FAU - Shoji, Takeshi
AU  - Shoji T
FAU - Okuda, Shinya
AU  - Okuda S
FAU - Izumi, Yoshihiro
AU  - Izumi Y
FAU - Fukusaki, Ei-ichiro
AU  - Fukusaki E
FAU - Kobayashi, Akio
AU  - Kobayashi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Caulimovirus/genetics
MH  - *Gene Transfer Techniques
MH  - Green Fluorescent Proteins/analysis/genetics
MH  - *Lasers
MH  - Microinjections/*methods
MH  - Microscopy, Electron, Scanning
MH  - Onions/chemistry/genetics/radiation effects
MH  - Plants/*radiation effects/ultrastructure
MH  - Promoter Regions, Genetic/genetics
EDAT- 2005/09/30 09:00
MHDA- 2006/04/19 09:00
CRDT- 2005/09/30 09:00
PHST- 2005/09/30 09:00 [pubmed]
PHST- 2006/04/19 09:00 [medline]
PHST- 2005/09/30 09:00 [entrez]
AID - 10.1002/bit.20709 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2006 Feb 5;93(2):325-31. doi: 10.1002/bit.20709.