PMID- 16193846
OWN - NLM
STAT- MEDLINE
DCOM- 20051027
LR  - 20191109
IS  - 0919-6544 (Print)
IS  - 0919-6544 (Linking)
VI  - 25
IP  - 3
DP  - 2005 Sep
TI  - Modified fluorescence in situ hybridization methods using pathology archives.
PG  - 269-73
AB  - Brain tumors, like many other common tumors, are frequently associated with 
      chromosomal numerical abnormalities. However, as the identification of abnormal 
      characteristics by conventional cytogenetic or molecular methods has been 
      hampered by technical difficulties, minimal information has been available about 
      specific chromosomal or locus-specific gene alterations. Recently, fluorescence 
      in situ hybridization (FISH) has emerged as a powerful clinical and research tool 
      for the assessment of genomic instability within interphase nuclei. Here, we 
      developed a modified FISH protocol including short-term microwave treatment to 
      analyze specimens from the pathology archives that had been routinely processed 
      and stored. The FISH signals obtained using this modified method showed a 
      significant improvement compared with those obtained using the standard FISH 
      method. This new technique thus enables the analysis of various paraffin-embedded 
      tissue sections of intracranial tumors obtained under inappropriate fixation 
      conditions. We highlight the advantages of this modified FISH procedure on a 
      tissue microarray of archival materials for current diagnostic and investigative 
      neuropathology applications.
FAU - Kitayama, Yasuhiko
AU  - Kitayama Y
AD  - Department of Pathology, Shizuoka Saiseikai General Hospital, Oshika, Shizuoka, 
      Japan. y142450@siz.saiseikai.or.jp
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Neuropathology
JT  - Neuropathology : official journal of the Japanese Society of Neuropathology
JID - 9606526
SB  - IM
MH  - Brain Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microwaves
MH  - *Paraffin Embedding
MH  - Tissue Array Analysis
EDAT- 2005/10/01 09:00
MHDA- 2005/10/28 09:00
CRDT- 2005/10/01 09:00
PHST- 2005/10/01 09:00 [pubmed]
PHST- 2005/10/28 09:00 [medline]
PHST- 2005/10/01 09:00 [entrez]
AID - 10.1111/j.1440-1789.2005.00628.x [doi]
PST - ppublish
SO  - Neuropathology. 2005 Sep;25(3):269-73. doi: 10.1111/j.1440-1789.2005.00628.x.