PMID- 16195216
OWN - NLM
STAT- MEDLINE
DCOM- 20051013
LR  - 20191210
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 68
IP  - 19
DP  - 2005 Oct 8
TI  - Age-related changes in oxidative DNA damage and benzo(a)pyrene diolepoxide-I 
      (BPDE-I)-DNA adduct levels in human stomach.
PG  - 1599-610
AB  - This study was conducted in order to obtain information on the association 
      between age and oxidative DNA damage and benzo[a]pyrene diolepoxide-I 
      (BPDE-I)-DNA adduct levels in the stomach tissues of normal subjects (n = 113). 
      Subjects ranged from 17 to 75 yr. The levels of 8-hydroxy-2'-deoxyguanosine 
      (8-OHdG), a biomarker of oxidative DNA damage, were measured by high-performance 
      liquid chromatography-electrochemical detector (HPLC-ECD). BPDE-I-DNA adduct 
      levels were quantitatively measured by enzyme-linked immunosorbent assay (ELISA) 
      using the monoclonal antibody 8E11. Increase in 8-OHdG levels was observed in 
      subjects from 31 to 60 yr of age. Higher levels of 8-OHdG were observed in the 
      stomach tissues of those in the 51-60 yr old group. Although Helicobacter. 
      Pylori-positive group subjects had higher 8-OHdG levels than those in the H. 
      pylori-negative group, no age-related changes in 8-OHdG levels were observed in 
      these groups. The levels of BPDE-I-DNA adduct also markedly increased with age. 
      Higher levels of BPDE-I-DNA adduct were observed in subjects aged 61-70 yr, but 
      this difference was not significant from other age groups. These results provide 
      evidence that there is a progressive age-dependent accumulation of oxidative DNA 
      damage and of BPDE-I-DNA adducts in human stomach tissues. It is possible that 
      such damage contributes to the known increased incidence of gastric tumor with 
      aging.
FAU - Lee, Byung Mu
AU  - Lee BM
AD  - Division of Toxicology, College of Pharmacy, Sungkyunkwan University, Changan-Ku, 
      Kyunggi-Do, Suwon.
FAU - Kwack, Seung Jun
AU  - Kwack SJ
FAU - Kim, Hyung Sik
AU  - Kim HS
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Benzopyrenes)
RN  - 0 (DNA Adducts)
RN  - 0 (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA)
RN  - 55097-80-8 (7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide)
RN  - 60268-85-1 (benzo(a)pyrene diolepoxide I)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/*metabolism
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Aging
MH  - Benzopyrenes/*metabolism
MH  - Child
MH  - Chromatography, High Pressure Liquid
MH  - DNA Adducts/*metabolism
MH  - DNA Damage
MH  - Deoxyguanosine/analogs & derivatives/metabolism
MH  - Female
MH  - Gastric Mucosa/*metabolism
MH  - Humans
MH  - Linear Models
MH  - Male
MH  - Middle Aged
MH  - Stomach Neoplasms/*metabolism
EDAT- 2005/10/01 09:00
MHDA- 2005/10/14 09:00
CRDT- 2005/10/01 09:00
PHST- 2005/10/01 09:00 [pubmed]
PHST- 2005/10/14 09:00 [medline]
PHST- 2005/10/01 09:00 [entrez]
AID - T2122N0227124261 [pii]
AID - 10.1080/15287390500182818 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2005 Oct 8;68(19):1599-610. doi: 
      10.1080/15287390500182818.