PMID- 16196107
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20191210
IS  - 0173-0835 (Print)
IS  - 1522-2683 (Electronic)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 19
DP  - 2005 Oct
TI  - Parallel mixing of photolithographically defined nanoliter volumes using 
      elastomeric microvalve arrays.
PG  - 3758-64
LID - 10.1002/elps.200500171
AB  - Portable microfluidic systems provide simple and effective solutions for low-cost 
      point-of-care diagnostics and high-throughput biomedical assays. Robust flow 
      control and precise fluidic volumes are two critical requirements for these 
      applications. We have developed a monolithic polydimethylsiloxane (PDMS) 
      microdevice that allows for storing and mixing subnanoliter volumes of aqueous 
      solutions at various mixing ratios. Filling and mixing is controlled via two 
      integrated PDMS microvalve arrays. The volumes of the microchambers are entirely 
      defined by photolithography, hence volumes from picoliter to nanoliter can be 
      fabricated with high precision. Because the microvalves do not require an energy 
      input to stay closed, fluid can be stored in a highly portable fashion for 
      several days. We have confirmed the mixing precision and predictability using 
      fluorescence microscopy. We also demonstrate the application of the device for 
      calibrating fluorescent calcium indicators. Due to the biocompatibility of PDMS, 
      the device will have broad applications in miniaturized diagnostic assays as well 
      as basic biological studies.
FAU - Li, Nianzhen
AU  - Li N
AD  - Department of Bioengineering, University of Washington, Seattle, WA 98195-2255, 
      USA. nianzhen@u.washington.edu
FAU - Hsu, Chia-Hsien
AU  - Hsu CH
FAU - Folch, Albert
AU  - Folch A
LA  - eng
GR  - R01 EB009761/EB/NIBIB NIH HHS/United States
GR  - R01 NS064387/NS/NINDS NIH HHS/United States
GR  - 61-0295/PHS HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Elastomers)
RN  - 0 (Polymers)
RN  - 0 (Silicones)
RN  - 0 (elastomeric polymer)
RN  - 63148-62-9 (baysilon)
RN  - SY7Q814VUP (Calcium)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Calcium/chemistry
MH  - Calibration
MH  - Dimethylpolysiloxanes/chemistry
MH  - Elastomers
MH  - Fluorescein/chemistry
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Microscopy, Fluorescence
MH  - Nanotechnology/*instrumentation
MH  - Polymers/chemistry
MH  - Silicones/chemistry
PMC - PMC3848874
MID - NIHMS473205
EDAT- 2005/10/01 09:00
MHDA- 2005/11/16 09:00
PMCR- 2013/12/03
CRDT- 2005/10/01 09:00
PHST- 2005/10/01 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/10/01 09:00 [entrez]
PHST- 2013/12/03 00:00 [pmc-release]
AID - 10.1002/elps.200500171 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 Oct;26(19):3758-64. doi: 10.1002/elps.200500171.