PMID- 16198261 OWN - NLM STAT- MEDLINE DCOM- 20051220 LR - 20171116 IS - 1389-1723 (Print) IS - 1347-4421 (Linking) VI - 100 IP - 2 DP - 2005 Aug TI - Identification of 2,4,6-trichloroanisole (TCA) causing a musty/muddy off-flavor in sake and its production in rice koji and moromi mash. PG - 178-83 AB - 2,4,6-Trichloroanisole (TCA), which has been identified as the main component responsible for the cork taint in wine, was detected in sake samples having a musty/muddy off-flavor by stir bar sorptive extraction (SBSE). We confirmed that TCA is one of the components causing this off-flavor in sake, as in other alcoholic beverages, from a sensory analysis showing the correlation between TCA concentration and the intensity of the musty/muddy off-flavor. We investigated the route of TCA production in the rice koji preparation process and in the moromi mash process for sake brewing. We found that TCA is produced mainly by the biomethylation of 2,4,6-trichlorophenol (TCP) by rice koji in brewing and that TCP originates from the wooden tools used in preparing rice koji. FAU - Miki, Atsushi AU - Miki A AD - National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-Hiroshima 739-0046, Japan. miki@kzkappa.co.jp FAU - Isogai, Atsuko AU - Isogai A FAU - Utsunomiya, Hitoshi AU - Utsunomiya H FAU - Iwata, Hiroshi AU - Iwata H LA - eng PT - Journal Article PL - Japan TA - J Biosci Bioeng JT - Journal of bioscience and bioengineering JID - 100888800 RN - 0 (Anisoles) RN - 31O3X41254 (2,4,6-trichloroanisole) SB - IM MH - Anisoles/*analysis MH - Calibration MH - Food Handling MH - Gas Chromatography-Mass Spectrometry MH - Oryza/*chemistry MH - Wine/*analysis EDAT- 2005/10/04 09:00 MHDA- 2005/12/21 09:00 CRDT- 2005/10/04 09:00 PHST- 2004/12/17 00:00 [received] PHST- 2005/04/20 00:00 [accepted] PHST- 2005/10/04 09:00 [pubmed] PHST- 2005/12/21 09:00 [medline] PHST- 2005/10/04 09:00 [entrez] AID - S1389-1723(05)70449-7 [pii] AID - 10.1263/jbb.100.178 [doi] PST - ppublish SO - J Biosci Bioeng. 2005 Aug;100(2):178-83. doi: 10.1263/jbb.100.178.