PMID- 16198307
OWN - NLM
STAT- MEDLINE
DCOM- 20051221
LR  - 20151119
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 337
IP  - 2
DP  - 2005 Nov 18
TI  - Store-operated Ca2+-channels are sensitive to changes in extracellular pH.
PG  - 571-9
AB  - The sensitivity of store-operated Ca(2+)-entry to changes in the extra- and 
      intracellular pH (pH(o) and pH(i), respectively) was investigated in SH-SY5Y 
      human neuroblastoma cells. The intracellular Ca(2+)-stores were depleted either 
      with 1 mM carbachol (CCH) or with 2 microM thapsigargin (TG). Extracellular 
      acidification suppressed both the CCH- and TG-mediated Ca(2+)-entry while 
      external alkalinization augmented both the CCH- and the TG-induced Ca(2+)-influx. 
      Mn(2+)-quenching experiments revealed that the rates of Ca(2+)-entry at the 
      thapsigargin- or carbachol-induced plateau were both accelerated at pH(o) 8.2 and 
      slowed down at pH(o) 6.8 with respect to the control at pH(o) 7.4. Alteration of 
      pH(o) between 6.8 and 8.2 did not have any significant prompt effect on pH(i) and 
      changes in pH(i) left the CCH-induced Ca(2+)-entry unaffected. These findings 
      demonstrate that physiologically relevant changes in pH(o) affect the 
      store-operated Ca(2+)-entry in SH-SY5Y cells and suggest that endogenous pH(o) 
      shifts may regulate cell activity in situ via modulating the store-operated 
      Ca(2+)-entry.
FAU - Laskay, G
AU  - Laskay G
AD  - Department of Botany, University of Szeged, Hungary. glaskay@bio.u-szeged.hu
FAU - Kalman, K
AU  - Kalman K
FAU - Van Kerkhove, E
AU  - Van Kerkhove E
FAU - Steels, P
AU  - Steels P
FAU - Ameloot, M
AU  - Ameloot M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050922
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Calcium Channels)
RN  - 0 (Cations, Divalent)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8Y164V895Y (Carbachol)
SB  - IM
MH  - Calcium Channels/*metabolism
MH  - Carbachol/pharmacology
MH  - Cations, Divalent
MH  - Cells, Cultured
MH  - Extracellular Space/drug effects/*metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Intracellular Space/metabolism
MH  - Kinetics
MH  - Manganese/metabolism/pharmacology
MH  - Thapsigargin/pharmacology
EDAT- 2005/10/04 09:00
MHDA- 2005/12/22 09:00
CRDT- 2005/10/04 09:00
PHST- 2005/08/19 00:00 [received]
PHST- 2005/09/09 00:00 [accepted]
PHST- 2005/10/04 09:00 [pubmed]
PHST- 2005/12/22 09:00 [medline]
PHST- 2005/10/04 09:00 [entrez]
AID - S0006-291X(05)02062-0 [pii]
AID - 10.1016/j.bbrc.2005.09.086 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2005 Nov 18;337(2):571-9. doi: 
      10.1016/j.bbrc.2005.09.086. Epub 2005 Sep 22.