PMID- 16198523
OWN - NLM
STAT- MEDLINE
DCOM- 20060518
LR  - 20220318
IS  - 0378-8741 (Print)
IS  - 0378-8741 (Linking)
VI  - 103
IP  - 2
DP  - 2006 Jan 16
TI  - Effects and mechanisms of crude astragalosides fraction on liver fibrosis in 
      rats.
PG  - 154-9
AB  - Astragalosides is the major active constituent of Radix Astragali. The present 
      study was carried out to investigate the effect of crude astragalosides fraction 
      (CAF) on rats liver fibrosis and its possible mechanisms. Hepatic fibrosis was 
      induced by subcutaneous injection with 50% CCl(4) in Sprague-Dawley rats. The 
      amount of CCl(4) administered was 1 mg kg(-1). The alanine aminotransferase 
      (ALT), aspartate aminotransferase (AST) levels in plasma and hydroxyproline 
      (Hyp), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione 
      peroxidase (GSH-px) contents in liver tissue were assayed by spectrophotometry. 
      The hyaluronic acid (HA) and procollagen III (PC III) were assessed by 
      radioimmunoassay. Tumor necrosis factor-alpha (TNF-alpha) and transforming growth 
      factor-beta1 (TGF-beta1) levels in culture supernatants of Kupffer cells (KCs) 
      were determined with ELISA. Liver samples collected after 8 weeks of CCl(4) 
      treatment were stained with hematoxylin-eosin (HE) and massion, and scored. 
      Intragastric administration of CAF (10, 20 and 40 mg kg(-1)) significantly 
      decreased indices of liver and spleen, the serum transaminase activities, HA and 
      PC III levels, and Hyp and MDA contents in liver tissue in rats of hepatic 
      fibrosis. Decreased SOD and GSH-px levels were reversed after administration of 
      CAF. Histopathological scores showed CAF had inhibitory effect on the progression 
      of hepatic fibrosis. In the in vitro experiments, CAF significantly reduced 
      TNF-alpha and TGF-beta1 levels in culture supernatants of KCs. The results showed 
      CAF significantly inhibited the progression of hepatic fibrosis induced by 
      CCl(4), and the inhibitory effect of CAF on hepatic fibrosis might be associated 
      with its ability to scavenge free radical and inhibit the production of TNF-alpha 
      and TGF-beta1 from activated KCs.
FAU - Gui, Shuang-Ying
AU  - Gui SY
AD  - Institute of Clinical Pharmacology, Anhui Medical University, Anhui Province, 
      Hefei 230032, PR China.
FAU - Wei, Wei
AU  - Wei W
FAU - Wang, Hua
AU  - Wang H
FAU - Wu, Li
AU  - Wu L
FAU - Sun, Wu-Yi
AU  - Sun WY
FAU - Chen, Wen-Bi
AU  - Chen WB
FAU - Wu, Cheng-Yi
AU  - Wu CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050929
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Plant Extracts)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 922OP8YUPF (Huang Qi)
SB  - IM
MH  - Animals
MH  - Astragalus propinquus
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Kupffer Cells/drug effects/metabolism
MH  - Liver Cirrhosis, Experimental/*drug therapy/enzymology/metabolism
MH  - Male
MH  - Plant Extracts/*therapeutic use
MH  - Plant Roots
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta/metabolism
MH  - Transforming Growth Factor beta1
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2005/10/04 09:00
MHDA- 2006/05/19 09:00
CRDT- 2005/10/04 09:00
PHST- 2005/04/07 00:00 [received]
PHST- 2005/07/08 00:00 [revised]
PHST- 2005/07/22 00:00 [accepted]
PHST- 2005/10/04 09:00 [pubmed]
PHST- 2006/05/19 09:00 [medline]
PHST- 2005/10/04 09:00 [entrez]
AID - S0378-8741(05)00515-5 [pii]
AID - 10.1016/j.jep.2005.07.025 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2006 Jan 16;103(2):154-9. doi: 10.1016/j.jep.2005.07.025. Epub 
      2005 Sep 29.