PMID- 16202029
OWN - NLM
STAT- MEDLINE
DCOM- 20051201
LR  - 20121115
IS  - 0909-8836 (Print)
IS  - 0909-8836 (Linking)
VI  - 113
IP  - 5
DP  - 2005 Oct
TI  - Inflammatogenic and adjuvant properties of HEMA in mice.
PG  - 410-6
AB  - 2-Hydroxyethylmethacrylate (HEMA), a common constituent in dental materials, is 
      known to cause hypersensitivity reactions. While the means by which this small 
      molecule causes adverse responses has not been ascertained, we have previously 
      demonstrated that it binds to protein and in mice induces the production of 
      autoantibodies to HEMA-conjugated self-protein. The present study explores the 
      inflammatory and adjuvant properties of HEMA in response to the subcutaneous 
      injection of HEMA and a protein. Ovalbumin (OVA) was used as a 'reporter 
      antigen', and mouse serum albumin (MSA), conjugated in vitro with HEMA (MSA(H)) 
      to a low degree (0.5 molecules of HEMA per molecule of MSA on average), was used 
      to mimic a possible in vivo situation. Inflammatory responses at injection sites 
      were scored by using an ordinal scale, and immunoglobulin (Ig)G1, IgG2a, and IgE 
      activities to OVA or MSA were assessed by enzyme-linked immunosorbent assay 
      (ELISA). Injections of 20 micromol HEMA induced overt inflammatory skin 
      responses, the severity of which was influenced by the co-administered 
      substances. A significantly higher IgG1 and IgE response to OVA was induced by 
      the presence of HEMA. Interestingly, injections with low conjugated MSA(H) only 
      induced the production of autoantibodies if free HEMA was included at the time of 
      immunization. These findings suggest that HEMA is an inflammatogenic substance 
      with adjuvant properties.
FAU - Sandberg, Elisabeth
AU  - Sandberg E
AD  - Section for Oral Immunology, Faculty of Odontology, the Sahlgrenska Academy at 
      Goteborg University, Goteborg, Sweden. Elisabeth.Sandberg@odontologi.gu.se
FAU - Kahu, Helena
AU  - Kahu H
FAU - Dahlgren, Ulf I
AU  - Dahlgren UI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies)
RN  - 0 (Autoantibodies)
RN  - 0 (Dental Materials)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Methacrylates)
RN  - 0 (Serum Albumin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 6E1I4IV47V (hydroxyethyl methacrylate)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Animals
MH  - Antibodies/immunology
MH  - Autoantibodies/immunology
MH  - Cells, Cultured
MH  - Dental Materials/chemistry/*pharmacology
MH  - Dermatitis/immunology
MH  - Female
MH  - Immunization
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Inflammation Mediators/*pharmacology
MH  - Injections, Subcutaneous
MH  - Methacrylates/chemistry/*pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/chemistry/immunology
MH  - Protein Binding
MH  - Serum Albumin/chemistry/immunology
MH  - Spleen/immunology/pathology
EDAT- 2005/10/06 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/06 09:00
PHST- 2005/10/06 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/06 09:00 [entrez]
AID - EOS234 [pii]
AID - 10.1111/j.1600-0722.2005.00234.x [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2005 Oct;113(5):410-6. doi: 10.1111/j.1600-0722.2005.00234.x.