PMID- 16203234
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20240515
IS  - 0091-6765 (Print)
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Linking)
VI  - 113
IP  - 10
DP  - 2005 Oct
TI  - Impact of polychlorinated biphenyls contamination on estrogenic activity in human 
      male serum.
PG  - 1277-84
AB  - Polychlorinated biphenyls (PCBs) are thought to cause numerous adverse health 
      effects, but their impact on estrogen signaling is still not fully understood. In 
      the present study, we used the ER-CALUX bioassay to determine 
      estrogenic/antiestrogenic activities of the prevalent PCB congeners and PCB 
      mixtures isolated from human male serum. The samples were collected from 
      residents of an area with an extensive environmental contamination from a former 
      PCB production site as well as from a neighboring background region in eastern 
      Slovakia. We found that the lower-chlorinated PCBs were estrogenic, whereas the 
      prevalent higher-chlorinated PCB congeners 138, 153, 170, 180, 187, 194, 199, and 
      203, as well as major PCB metabolites, behaved as antiestrogens. Coplanar PCBs 
      had no direct effect on estrogen receptor (ER) activation in this in vitro model. 
      In human male serum samples, high levels of PCBs were associated with a decreased 
      ER-mediated activity and an increased dioxin-like activity, as determined by the 
      DR-CALUX assay. 17beta-Estradiol (E2) was responsible for a major part of 
      estrogenic activity identified in total serum extracts. Significant negative 
      correlations were found between dioxin-like activity, as well as mRNA levels of 
      cytochromes P450 1A1 and 1B1 in lymphocytes, and total estrogenic activity. For 
      sample fractions containing only persistent organic pollutants (POPs), the 
      increased frequency of antiestrogenic samples was associated with a higher sum of 
      PCBs. This suggests that the prevalent non-dioxin-like PCBs were responsible for 
      the weak antiestrogenic activity of some POPs fractions. Our data also suggest 
      that it might be important to pay attention to direct effects of PCBs on steroid 
      hormone levels in heavily exposed subjects.
FAU - Pliskova, Martina
AU  - Pliskova M
AD  - Veterinary Research Institute, Brno, Czech Republic.
FAU - Vondracek, Jan
AU  - Vondracek J
FAU - Canton, Rocio Fernandez
AU  - Canton RF
FAU - Nera, Jiri
AU  - Nera J
FAU - Kocan, Anton
AU  - Kocan A
FAU - Petrik, Jan
AU  - Petrik J
FAU - Trnovec, Tomas
AU  - Trnovec T
FAU - Sanderson, Thomas
AU  - Sanderson T
FAU - van den Berg, Martin
AU  - van den Berg M
FAU - Machala, Miroslav
AU  - Machala M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Environmental Pollutants)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 4TI98Z838E (Estradiol)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Environmental Pollutants/analysis/*toxicity
MH  - Estradiol/*blood
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Male
MH  - Polychlorinated Biphenyls/analysis/*toxicity
MH  - Receptors, Aryl Hydrocarbon/agonists
MH  - Slovakia
PMC - PMC1281266
EDAT- 2005/10/06 09:00
MHDA- 2006/01/24 09:00
PMCR- 2005/10/01
CRDT- 2005/10/06 09:00
PHST- 2005/10/06 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/10/06 09:00 [entrez]
PHST- 2005/10/01 00:00 [pmc-release]
AID - ehp0113-001277 [pii]
AID - 10.1289/ehp.7745 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2005 Oct;113(10):1277-84. doi: 10.1289/ehp.7745.