PMID- 16204378
OWN - NLM
STAT- MEDLINE
DCOM- 20060420
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 102
IP  - 41
DP  - 2005 Oct 11
TI  - Sex differences in estrogenic regulation of neuronal activity in neonatal 
      cultures of ventromedial nucleus of the hypothalamus.
PG  - 14907-12
AB  - Estrogenic effects have been implicated in sexual differentiation of brain and 
      behavior, in part by affecting neuronal activity in the ventromedial nucleus of 
      the hypothalamus (VMN). We report here a remarkable sex difference in estrogenic 
      regulation of neuronal activity in male vs. female neural networks. Spontaneous 
      synaptic currents originating from a population of neurons were recorded in 
      primary VMN cultures using the whole-cell patch-clamp technique. Treatment with 
      17beta-estradiol (E2, 10 nM) for 24 h induced opposite effects in the two sexes: 
      the frequency of spontaneous synaptic events decreased significantly in neurons 
      derived from males but increased in those from females. Interestingly, the 
      24-hour E2 effect was partially reversed by an acute application (5 min) of a 
      second dose of E2 (10 nM), suggesting an interaction between extended (24-hr) and 
      acute (5-min) effects of E2 in VMN neurons. To understand the underlying 
      mechanism of this sexually dimorphic action of E2, we analyzed the E2 effect on 
      GABAergic neurotransmission by recording miniature inhibitory postsynaptic 
      currents. After 24-hour E2 treatment, both the amplitude and frequency of 
      miniature inhibitory postsynaptic currents increased in neurons derived from 
      males but decreased in those from females. These results suggest that E2-induced 
      changes in GABAergic inhibition could at least partially explain E2 effects on 
      neuronal activity. We conclude that E2 may have sexually dimorphic effects on the 
      synaptic output of VMN neurons by modulating GABAergic neurotransmission.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Laboratory of Neurobiology and Behavior, The Rockefeller University, New York, NY 
      10021, USA. zhouj@rockefeller.edu
FAU - Pfaff, Donald W
AU  - Pfaff DW
FAU - Chen, Gong
AU  - Chen G
LA  - eng
GR  - R01 HD005751/HD/NICHD NIH HHS/United States
GR  - R37 HD005751/HD/NICHD NIH HHS/United States
GR  - HD-05751/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20051004
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Male
MH  - Neurons/drug effects/*metabolism
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
MH  - Synaptic Transmission/*drug effects
MH  - Time Factors
MH  - Ventromedial Hypothalamic Nucleus/drug effects/*metabolism
PMC - PMC1253608
EDAT- 2005/10/06 09:00
MHDA- 2006/04/21 09:00
PMCR- 2006/04/11
CRDT- 2005/10/06 09:00
PHST- 2005/10/06 09:00 [pubmed]
PHST- 2006/04/21 09:00 [medline]
PHST- 2005/10/06 09:00 [entrez]
PHST- 2006/04/11 00:00 [pmc-release]
AID - 0507440102 [pii]
AID - 010214907 [pii]
AID - 10.1073/pnas.0507440102 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14907-12. doi: 
      10.1073/pnas.0507440102. Epub 2005 Oct 4.