PMID- 16204888
OWN - NLM
STAT- MEDLINE
DCOM- 20051123
LR  - 20070724
IS  - 0907-4449 (Print)
IS  - 0907-4449 (Linking)
VI  - 61
IP  - Pt 10
DP  - 2005 Oct
TI  - Three-dimensional structure determination of proteins related to human health in 
      their functional context at The Israel Structural Proteomics Center (ISPC). This 
      paper was presented at ICCBM10.
PG  - 1364-72
AB  - The principal goal of the Israel Structural Proteomics Center (ISPC) is to 
      determine the structures of proteins related to human health in their functional 
      context. Emphasis is on the solution of structures of proteins complexed with 
      their natural partner proteins and/or with DNA. To date, the ISPC has solved the 
      structures of 14 proteins, including two protein complexes. It has adopted 
      automated high-throughput (HTP) cloning and expression techniques and is now 
      expressing in Escherichia coli, Pichia pastoris and baculovirus, and in a 
      cell-free E. coli system. Protein expression in E. coli is the primary system of 
      choice in which different parameters are tested in parallel. Much effort is being 
      devoted to development of automated refolding of proteins expressed as inclusion 
      bodies in E. coli. The current procedure utilizes tagged proteins from which the 
      tag can subsequently be removed by TEV protease, thus permitting streamlined 
      purification of a large number of samples. Robotic protein crystallization 
      screens and optimization utilize both the batch method under oil and vapour 
      diffusion. In order to record and organize the data accumulated by the ISPC, a 
      laboratory information-management system (LIMS) has been developed which 
      facilitates data monitoring and analysis. This permits optimization of conditions 
      at all stages of protein production and structure determination. A set of 
      bioinformatics tools, which are implemented in our LIMS, is utilized to analyze 
      each target.
FAU - Albeck, Shira
AU  - Albeck S
AD  - Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, 
      Israel.
FAU - Burstein, Yigal
AU  - Burstein Y
FAU - Dym, Orly
AU  - Dym O
FAU - Jacobovitch, Yossi
AU  - Jacobovitch Y
FAU - Levi, Nurit
AU  - Levi N
FAU - Meged, Ran
AU  - Meged R
FAU - Michael, Yigal
AU  - Michael Y
FAU - Peleg, Yoav
AU  - Peleg Y
FAU - Prilusky, Jaime
AU  - Prilusky J
FAU - Schreiber, Gideon
AU  - Schreiber G
FAU - Silman, Israel
AU  - Silman I
FAU - Unger, Tamar
AU  - Unger T
FAU - Sussman, Joel L
AU  - Sussman JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050928
PL  - United States
TA  - Acta Crystallogr D Biol Crystallogr
JT  - Acta crystallographica. Section D, Biological crystallography
JID - 9305878
RN  - 0 (Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Automation
MH  - Cloning, Molecular
MH  - Computational Biology/methods
MH  - DNA/chemistry
MH  - *Databases, Protein
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Escherichia coli/metabolism
MH  - Humans
MH  - Inclusion Bodies
MH  - Internet
MH  - Israel
MH  - Models, Molecular
MH  - Pichia/metabolism
MH  - Protein Conformation
MH  - Protein Denaturation
MH  - Protein Folding
MH  - Proteins/*chemistry
MH  - Proteomics/*methods
MH  - Recombinant Proteins/chemistry
EDAT- 2005/10/06 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/06 09:00
PHST- 2004/09/07 00:00 [received]
PHST- 2005/07/22 00:00 [accepted]
PHST- 2005/10/06 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/06 09:00 [entrez]
AID - S0907444905023565 [pii]
AID - 10.1107/S0907444905023565 [doi]
PST - ppublish
SO  - Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1364-72. doi: 
      10.1107/S0907444905023565. Epub 2005 Sep 28.