PMID- 16206670
OWN - NLM
STAT- MEDLINE
DCOM- 20060609
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 40
IP  - 12
DP  - 2001 Dec
TI  - [Effects of glutamine on the intestinal failure in rats model of acute 
      necrotizing pancreatitis].
PG  - 815-8
AB  - OBJECTIVE: To determine the effects of glutamine (Gln) on the intestinal failure 
      in rats with acute necrotizing pancreatitis (ANP) and its possible mechanisms. 
      METHODS: Fifty-four Sprague-Dawley rats were randomly divided into 3 groups: sham 
      operation (SO, n = 18), ANP (n = 18), and ANP treated with Gln (ANP + Gln, n = 
      18). ANP model was induced by injection of 5% sodium taurocholate solution into 
      bilo-pancreatic duct. The therapy was continuously given with amino acid solution 
      by a mini-pump via a central intravenous line. In addition, the ANP + Gin group 
      was received 3% Gln dipeptide solution (equal to 2% Gln) with a dosage of 0.5g x 
      kg(-1) x d(-1). These groups were isocaloric and isonitrogenous. Bacterial 
      cultures from pancreas, mesenteric lymph node (MLN), liver, spleen and ascites 
      were done at 24, 48, 72 h after operation. Endotoxin level in portal vein was 
      determined. Pathologic changes of intestinal mucosa were also studied. Apoptosis 
      of intestinal mucosa was determined by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) method. Expressions of 
      insulin-like growth factor 1 (IGF-1), Gln synthetase (GSase) and glutaminase 
      (Glnase) mRNA were assayed by reverse transcription polymerase chain reaction 
      (RT-PCR). RESULTS: At 24, 48, 72h, the positive rate of bacterial culture and the 
      endotoxin concentration were increased significantly in ANP group compared to the 
      SO group (P < 0.05), while Gln could decrease them significantly. Pathologic 
      study showed that the height of mucosal villous in ANP group was lower than that 
      in SO group, indicating the intestinal mucosa became more atrophy. However, the 
      height of mucosal villous in ANP + Gln group was no significantly difference 
      compared to that in SO group, indicated Gln could preserve the mucosa well. 
      Apoptotic index was increased in ANP group and decreased in Gln treated rats. 
      Expressions of IGF-1, GSase, Glnase mRNA were down-regulated in ANP group, but 
      were up-regulated in ANP + Gln group. CONCLUSIONS: The intestinal barrier 
      function was impaired in ANP. Gln could protect intestinal barrier function. This 
      action was probably related to its enhancement of IGF-1, GSase and Glnase mRNA 
      expressions and its inhibition of intestinal mucosal apoptosis.
FAU - Wang, X
AU  - Wang X
AD  - Department of Gastroenterology, Shanghai First People's Hospital, Shanghai 
      200080, China.
FAU - Wu, K
AU  - Wu K
FAU - Wang, B
AU  - Wang B
FAU - Xu, X
AU  - Xu X
FAU - Xu, M
AU  - Xu M
FAU - Gong, Z
AU  - Gong Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (RNA, Messenger)
RN  - 0RH81L854J (Glutamine)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - EC 3.5.1.2 (Glutaminase)
RN  - EC 6.3.1.2 (Glutamate-Ammonia Ligase)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Down-Regulation
MH  - Glutamate-Ammonia Ligase/genetics/metabolism
MH  - Glutaminase/genetics/metabolism
MH  - Glutamine/*pharmacology
MH  - Insulin-Like Growth Factor I/genetics/metabolism
MH  - Intestinal Mucosa/pathology/*physiopathology
MH  - Male
MH  - Pancreatitis, Acute Necrotizing/pathology/*physiopathology
MH  - RNA, Messenger/genetics
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
EDAT- 2005/10/07 09:00
MHDA- 2006/06/10 09:00
CRDT- 2005/10/07 09:00
PHST- 2005/10/07 09:00 [pubmed]
PHST- 2006/06/10 09:00 [medline]
PHST- 2005/10/07 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2001 Dec;40(12):815-8.