PMID- 16207215
OWN - NLM
STAT- MEDLINE
DCOM- 20051213
LR  - 20091103
IS  - 0009-9163 (Print)
IS  - 0009-9163 (Linking)
VI  - 68
IP  - 5
DP  - 2005 Nov
TI  - Evidence for deletion of 9q as a two-step process in chronic myeloid leukemia.
PG  - 461-5
AB  - Evidence for deletion of 9q as a two-step process in chronic myeloid leukemia. 
      Chronic myeloid leukemia (CML) is characterized by the Philadelphia translocation 
      t(9;22)(q34;q11) resulting in the BCR/ABL fusion gene. Submicroscopic deletion of 
      the derivative chromosome 9 occurs in a subset of these patients and is 
      associated with poor prognosis. In the current study, we present two unusual 
      cases of CML selected from a series of 54 consecutive cases. A detailed study 
      using classical cytogenetics and fluorescence in situ hybridization (FISH) 
      analysis was done using dual color extra signal FISH and whole chromosome paint 
      in order to elucidate the mechanism of 9q deletion. One case had two clones on 
      interphase FISH, one with and one without chromosome 9q deletion. The other case 
      had two clones on both cytogenetic and FISH analyses, one with and one without a 
      marker chromosome carrying chromosome 9q sequences. In this latter case, the 
      clone with deletion of the derivative chromosome 9 comprised 21.1% at diagnosis, 
      increasing to 36.8% after 11 months, suggesting a growth advantage. We report 
      here evidence that deletions on 9q in CML may occur through breakage and 
      rearrangement of chromosomes resulting in derivative chromosomes and either a 
      marker chromosome or fragment/episome, followed by loss of chromosome material 
      from the cell.
FAU - Rudduck-Sivaswaren, C
AU  - Rudduck-Sivaswaren C
AD  - Department of Pathology, Singapore General Hospital, Singapore.
FAU - Tien, S-L
AU  - Tien SL
FAU - Lim, P
AU  - Lim P
FAU - Lim, E
AU  - Lim E
FAU - Lie, D K H
AU  - Lie DK
FAU - Tan, P H C
AU  - Tan PH
FAU - Lee, A S G
AU  - Lee AS
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Clin Genet
JT  - Clinical genetics
JID - 0253664
SB  - IM
MH  - Adult
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Translocation, Genetic
EDAT- 2005/10/07 09:00
MHDA- 2005/12/15 09:00
CRDT- 2005/10/07 09:00
PHST- 2005/10/07 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/10/07 09:00 [entrez]
AID - CGE521 [pii]
AID - 10.1111/j.1399-0004.2005.00521.x [doi]
PST - ppublish
SO  - Clin Genet. 2005 Nov;68(5):461-5. doi: 10.1111/j.1399-0004.2005.00521.x.