PMID- 16207252
OWN - NLM
STAT- MEDLINE
DCOM- 20051129
LR  - 20220417
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 7
IP  - 11
DP  - 2005 Nov
TI  - A novel CD4-CD8alpha+CD205+CD11b- murine spleen dendritic cell line: 
      establishment, characterization and functional analysis in a model of vaccination 
      to toxoplasmosis.
PG  - 1659-71
AB  - Dendritic cells (DCs) play an essential role in the induction of immune responses 
      to pathogen infections. Native DCs are difficult to obtain in large numbers and 
      consequently the vast majority of DCs employed in all experiments are derived 
      from bone marrow progenitors. In an attempt to solve this problem, we have 
      established a novel CD8alpha(+) DC line (H-2(k)) from spleen, which we have named 
      SRDC line, and which is easy to culture in vitro. These cells display similar 
      morphology, phenotype and activity to CD4(-)CD8alpha(+)CD205(+)CD11b(-) DCs 
      purified ex vivo. Toxoplasma gondii antigen was shown to be taken up by these 
      cells and to increase class I and class II major histocompatibility complex 
      (MHC), CD40, CD80 and CD86 surface expression. We report that vaccination with T. 
      gondii antigen-pulsed SRDCs, which synthesize large amounts of interleukin-12, 
      induced protective immune responses against this intracellular pathogen in 
      syngeneic CBA/J mice. This protection was associated with strong cellular and 
      humoral immune responses at systemic and intestinal levels. Spleen and mesenteric 
      lymph node cell proliferations were correlated with a Th1/Th2-type response and a 
      specific SRDC homing to spleen and intestine was observed. The SRDC or 
      CD4(-)CD8alpha(+)CD205(+)CD11b(-) DC line can be expected to be a very useful 
      tool for immunobiology studies of DC.
FAU - Ruiz, Sophie
AU  - Ruiz S
AD  - Universite Francois-Rabelais de Tours, Tours, France.
FAU - Beauvillain, Celine
AU  - Beauvillain C
FAU - Mevelec, Marie-Noelle
AU  - Mevelec MN
FAU - Roingeard, Philippe
AU  - Roingeard P
FAU - Breton, Pascal
AU  - Breton P
FAU - Bout, Daniel
AU  - Bout D
FAU - Dimier-Poisson, Isabelle
AU  - Dimier-Poisson I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Protozoan Vaccines)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Protozoan/immunology
MH  - CD11b Antigen/metabolism
MH  - CD4 Antigens/metabolism
MH  - CD8 Antigens/metabolism
MH  - Cell Line
MH  - Dendritic Cells/*immunology/metabolism/*parasitology
MH  - Lectins, C-Type/metabolism
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Minor Histocompatibility Antigens
MH  - Protozoan Vaccines/*administration & dosage/immunology
MH  - Receptors, Cell Surface/metabolism
MH  - Spleen/cytology
MH  - Toxoplasma/*immunology
MH  - Toxoplasmosis/*immunology/prevention & control
MH  - Vaccination
EDAT- 2005/10/07 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/07 09:00
PHST- 2005/10/07 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/07 09:00 [entrez]
AID - CMI583 [pii]
AID - 10.1111/j.1462-5822.2005.00583.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2005 Nov;7(11):1659-71. doi: 10.1111/j.1462-5822.2005.00583.x.