PMID- 16207523
OWN - NLM
STAT- MEDLINE
DCOM- 20060426
LR  - 20091119
IS  - 0739-7240 (Print)
IS  - 0739-7240 (Linking)
VI  - 30
IP  - 3
DP  - 2006 Mar
TI  - Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and 
      expression analysis in the mammary gland.
PG  - 239-46
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that 
      plays a crucial role in the embryonic and postnatal development of various organs 
      including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, 
      and examined their expression during mammary gland development in dairy cows. The 
      2.5-kbp HGF cDNA clone contained a 2190 bp open reading frame coding a 730 amino 
      acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4152 bp open reading 
      frame coding a 1384 amino acid protein. The bovine HGF and c-Met sequences 
      exhibited more than 87% identity with those of other mammals. RT-PCR analysis 
      revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine 
      tissues tested. HGF mRNA was detected only in the inactive stage of bovine 
      mammary gland development and not in the developing, lactating, and involuting 
      stages, while c-Met mRNA was detected in the inactive and involuting stages. 
      Immunohistochemical analysis demonstrated that the c-Met protein was found on 
      mammary epithelial cells in the inactive, developing, and involuting stages, and 
      on myoepithelial cells in all stages. These results suggest pivotal roles of HGF 
      and c-Met in the development of bovine mammary gland.
FAU - Yamaji, Daisuke
AU  - Yamaji D
AD  - Laboratory of Biochemistry, Department of Biomedical Sciences, Graduate School of 
      Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
FAU - Kimura, Kazuhiro
AU  - Kimura K
FAU - Watanabe, Atsushi
AU  - Watanabe A
FAU - Kon, Yasuhiro
AU  - Kon Y
FAU - Iwanaga, Toshihiko
AU  - Iwanaga T
FAU - Soliman, Mohamed M
AU  - Soliman MM
FAU - Ahmed, Mohamed M
AU  - Ahmed MM
FAU - Saito, Masayuki
AU  - Saito M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050921
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (RNA, Messenger)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Female
MH  - Gene Expression
MH  - Hepatocyte Growth Factor/biosynthesis/*genetics
MH  - Immunohistochemistry
MH  - Lactation
MH  - Mammary Glands, Animal/growth & development/metabolism/*physiology
MH  - Proto-Oncogene Proteins c-met/*genetics
MH  - RNA, Messenger/biosynthesis/chemistry/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
EDAT- 2005/10/07 09:00
MHDA- 2006/04/28 09:00
CRDT- 2005/10/07 09:00
PHST- 2005/06/20 00:00 [received]
PHST- 2005/08/22 00:00 [revised]
PHST- 2005/08/25 00:00 [accepted]
PHST- 2005/10/07 09:00 [pubmed]
PHST- 2006/04/28 09:00 [medline]
PHST- 2005/10/07 09:00 [entrez]
AID - S0739-7240(05)00185-2 [pii]
AID - 10.1016/j.domaniend.2005.08.005 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2006 Mar;30(3):239-46. doi: 
      10.1016/j.domaniend.2005.08.005. Epub 2005 Sep 21.