PMID- 16209128
OWN - NLM
STAT- MEDLINE
DCOM- 20051025
LR  - 20190608
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 24
IP  - 3
DP  - 2004 Jun
TI  - The sensitizers nickel sulfate and 2,4-dinitrofluorobenzene increase CD40 and 
      IL-12 receptor expression in a fetal skin dendritic cell line.
PG  - 191-202
AB  - Dendritic cells (DCs) are antigen-presenting cells (APCs) capable of capturing 
      haptens and to process and present them to T lymphocytes. In order to sensitize T 
      cells for contact hypersensitivity (CHS), skin DCs suffer a maturation process 
      with modifications on their surface molecules. The aim of this work was to 
      evaluate changes induced by two contact sensitizers, 2,4-dinitrofluorobenzene 
      (DNFB) and nickel sulfate (NiSO4), and a non-sensitizer 2,4-dichloronitrobenzene 
      (DCNB), on the protein levels of two activation markers, CD40 and IL-12 receptor 
      (IL-12R), in a mouse skin dendritic cell line (FSDC). The expression of CD40 and 
      IL-12R proteins was evaluated by western blot assay and direct immunofluorescence 
      microscopy. The results showed that CD40 and IL-12R expression increased 
      significantly after cell exposure to NiSO4 and DNFB, although DNFB exhibited a 
      stronger activity. There was no effect with DCNB. The epidermal cytokine 
      granulocyte-macrophage colony-stimulating factor (GM-CSF), also used in the 
      experiments, slightly increased the expression of both CD40 and IL-12R and when 
      tested together with the sensitizers the effect was partially additive. The 
      results suggest that the sensitizers DNFB and NiSO4 are directly involved on the 
      changes of the surface markers CD40 and IL-12R in skin DCs, during the 
      sensitization phase of CHS, and this effect may be enhanced by GM-CSF. In 
      contrast, no effect was observed with DCNB.
FAU - Vital, Ana Luisa
AU  - Vital AL
AD  - Faculdade de Farmacia, Universidade de Coimbra, Rua do Norte, 3000-295, Coimbra, 
      Portugal.
FAU - Goncalo, Margarida
AU  - Goncalo M
FAU - Cruz, Maria Teresa
AU  - Cruz MT
FAU - Figueiredo, Americo
AU  - Figueiredo A
FAU - Duarte, Carlos B
AU  - Duarte CB
FAU - Celeste Lopes, Maria
AU  - Celeste Lopes M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (CD40 Antigens)
RN  - 0 (Irritants)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-12)
RN  - 4FLT4T3WUN (nickel sulfate)
RN  - 7OV03QG267 (Nickel)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - CD40 Antigens/*metabolism
MH  - Cell Line
MH  - *Dendritic Cells/cytology/drug effects/metabolism
MH  - Dermatitis, Contact
MH  - Dinitrofluorobenzene/*pharmacology
MH  - Fetus/*anatomy & histology/physiology
MH  - Irritants/pharmacology
MH  - Mice
MH  - Nickel/*pharmacology
MH  - Receptors, Interleukin/*metabolism
MH  - Receptors, Interleukin-12
MH  - *Skin/cytology/drug effects
EDAT- 2005/10/08 09:00
MHDA- 2005/10/26 09:00
CRDT- 2005/10/08 09:00
PHST- 2005/10/08 09:00 [pubmed]
PHST- 2005/10/26 09:00 [medline]
PHST- 2005/10/08 09:00 [entrez]
AID - 10.1007/s10540-005-2580-7 [doi]
PST - ppublish
SO  - Biosci Rep. 2004 Jun;24(3):191-202. doi: 10.1007/s10540-005-2580-7.