PMID- 16210412
OWN - NLM
STAT- MEDLINE
DCOM- 20060209
LR  - 20111117
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 23
IP  - 9
DP  - 2005 Oct
TI  - Human cord blood--derived cells generate insulin-producing cells in vivo.
PG  - 1409-16
AB  - Here we report the capacity of human cord blood (CB)-derived cells to generate 
      insulin-producing cells. To investigate in vivo capacity of human CB-derived 
      cells, T cell-depleted mononuclear cells were intravenously transplanted into 
      nonobese diabetic/severe combined immunodeficient/beta2-microglobulinnull mice 
      within 48 hours of birth. At 1-2 months post-transplantation, immunofluorescence 
      staining for insulin and fluorescence in situ hybridization (FISH) analysis using 
      a human chromosome probe indicated that human CB-derived cells generated 
      insulin-producing cells at a frequency of 0.65%+/-0.64% in xenogeneic hosts. 
      Reverse transcription-polymerase chain reaction analysis confirmed the 
      transcription of human insulin in the pancreatic tissue of the recipient mice. To 
      clarify the mechanism underlying CB-derived insulin-producing cells, double FISH 
      analysis using species-specific probes was performed. Almost equal proportions of 
      human chromosome+ murine chromosome- insulin+ cells and human chromosome+ murine 
      chromosome+ insulin+ cells were present in recipient pancreatic islets. Taken 
      together, human CB contains progenitor cells, which can generate 
      insulin-producing cells in recipient pancreatic tissues across a xenogeneic 
      histocompatibility barrier by fusion-dependent and -independent mechanisms.
FAU - Yoshida, Shuro
AU  - Yoshida S
AD  - Department of Medicine and Biosystemic Science, Kyushu University Graduate School 
      of Medicine, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
FAU - Ishikawa, Fumihiko
AU  - Ishikawa F
FAU - Kawano, Noriaki
AU  - Kawano N
FAU - Shimoda, Kazuya
AU  - Shimoda K
FAU - Nagafuchi, Seiho
AU  - Nagafuchi S
FAU - Shimoda, Shinji
AU  - Shimoda S
FAU - Yasukawa, Masaki
AU  - Yasukawa M
FAU - Kanemaru, Takaaki
AU  - Kanemaru T
FAU - Ishibashi, Hiromi
AU  - Ishibashi H
FAU - Shultz, Leonard D
AU  - Shultz LD
FAU - Harada, Mine
AU  - Harada M
LA  - eng
GR  - AI30389/AI/NIAID NIH HHS/United States
GR  - DK52530/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Insulin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - *Cord Blood Stem Cell Transplantation
MH  - Fetal Blood/cytology
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Insulin/*biosynthesis/genetics
MH  - Islets of Langerhans/*cytology/metabolism
MH  - Leukocytes, Mononuclear/cytology/metabolism/*transplantation
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
MH  - *Transplantation, Heterologous
EDAT- 2005/10/08 09:00
MHDA- 2006/02/10 09:00
CRDT- 2005/10/08 09:00
PHST- 2005/10/08 09:00 [pubmed]
PHST- 2006/02/10 09:00 [medline]
PHST- 2005/10/08 09:00 [entrez]
AID - 23/9/1409 [pii]
AID - 10.1634/stemcells.2005-0079 [doi]
PST - ppublish
SO  - Stem Cells. 2005 Oct;23(9):1409-16. doi: 10.1634/stemcells.2005-0079.