PMID- 16210484
OWN - NLM
STAT- MEDLINE
DCOM- 20051214
LR  - 20181113
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 12
IP  - 10
DP  - 2005 Oct
TI  - Generation of feline dendritic cells derived from peripheral blood monocytes for 
      in vivo use.
PG  - 1202-8
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that can prime T 
      cells and polarize the cellular immune response. Because Th1-type immune 
      responses have been connected to success in combating viral infection, a 
      promising therapeutic application of DCs would be their differentiation in vitro 
      and injection back into the host to boost an immune response in infected animals. 
      This study was aimed both at developing a protocol to cultivate feline DCs in the 
      absence of exogenous proteins for their use in vivo and at investigating what 
      might be the most appropriate stimulus to induce their maturation in vitro and 
      finding correlates of maturation. We generated DCs from peripheral blood 
      monocytes in the presence of feline interleukin-4 and granulocyte-macrophage 
      colony stimulating factor, and after 5 days their maturation was induced with 
      either lipopolysaccharide, human recombinant tumor necrosis factor alpha, 
      poly(I:C), or activated feline platelets. After 48 h, their CD14, CD1a, major 
      histocompatibility complex class II, and B7.1 surface expression was analyzed in 
      parallel with their ability to uptake antigen or prime a mixed leukocyte 
      reaction. The results presented show that feline DCs cultured in autologous 
      plasma differentiate and are able to mature in the presence of stimuli similar to 
      the ones currently used for other species. The present work sets the grounds for 
      future use of DCs obtained by the protocol described for in vivo vaccination and 
      immunotherapy of feline immunodeficiency virus-infected cats.
FAU - Freer, Giulia
AU  - Freer G
AD  - Retrovirus Center and Virology Section, Department of Experimental Pathology, 
      University of Pisa, Via Del Brennero 2, I-56127 Pisa, Italy. 
      freer@biomed.unipi.it
FAU - Matteucci, Donatella
AU  - Matteucci D
FAU - Mazzetti, Paola
AU  - Mazzetti P
FAU - Bozzacco, Leonia
AU  - Bozzacco L
FAU - Bendinelli, Mauro
AU  - Bendinelli M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (Antigens, Surface)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, Surface/analysis
MH  - Blood Cells/cytology
MH  - Cats
MH  - Cell Culture Techniques
MH  - Culture Media
MH  - Cytokines
MH  - Dendritic Cells/*cytology/immunology
MH  - Female
MH  - Immunophenotyping
MH  - Methods
MH  - Monocytes/cytology
PMC - PMC1247835
EDAT- 2005/10/08 09:00
MHDA- 2005/12/15 09:00
PMCR- 2005/10/01
CRDT- 2005/10/08 09:00
PHST- 2005/10/08 09:00 [pubmed]
PHST- 2005/12/15 09:00 [medline]
PHST- 2005/10/08 09:00 [entrez]
PHST- 2005/10/01 00:00 [pmc-release]
AID - 12/10/1202 [pii]
AID - 0177-05 [pii]
AID - 10.1128/CDLI.12.10.1202-1208.2005 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2005 Oct;12(10):1202-8. doi: 
      10.1128/CDLI.12.10.1202-1208.2005.