PMID- 16211234
OWN - NLM
STAT- MEDLINE
DCOM- 20051215
LR  - 20111117
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 27
IP  - 5
DP  - 2005 Nov
TI  - Identification of human papillomavirus 16-E6 protein-derived peptides with the 
      potential to generate cytotoxic T-lymphocytes toward human leukocyte antigen-A24+ 
      cervical cancer.
PG  - 1371-9
AB  - Human papillomavirus 16 (HPV16)-E6 and -E7 proteins are considered to be 
      appropriate targets in specific immunotherapy for cervical cancer. In this study, 
      we attempted to identify epitope peptides from the HPV16-E6 protein that have the 
      potential to generate cytotoxic T-lymphocytes (CTLs) toward human leukocyte 
      antigen (HLA)-A24+ cervical cancer. Two HPV16-E6 peptides at positions 75-83 and 
      91-100 effectively induced peptide-specific CTLs from peripheral blood 
      mononuclear cells of HLA-A24+ cervical cancer patients. These HPV16-E6 
      peptide-induced CTLs showed cytotoxicity against HLA-A24+ and HPV16-E6 
      protein-expressing cervical cancer cells. Experiments with blocking antibodies 
      and cold inhibition targets revealed that the cytotoxicity was mainly dependent 
      on peptide-specific and CD8+ T cells. In addition, based on our observation that 
      induction of immunoglobulin G (IgG) reactive to administered CTL-directed 
      peptides is correlated with clinical responses, we attempted to detect IgG 
      reactive to HPV16-E6 peptides in the plasma of cancer patients. As a result, IgGs 
      reactive to the HPV16-E691-100 peptide were detected in 4 of 12 cervical cancer 
      patients. These results indicate that these HPV16-E6-derived peptides are good 
      candidates in peptide-based immunotherapy for HLA-A24+ cervical cancer patients.
FAU - Hara, Miho
AU  - Hara M
AD  - Department of Immunology, Kurume University School of Medicine, Kurume, Fukuoka 
      830-0011, Japan.
FAU - Matsueda, Satoko
AU  - Matsueda S
FAU - Tamura, Mayumi
AU  - Tamura M
FAU - Takedatsu, Hiroko
AU  - Takedatsu H
FAU - Tanaka, Masahiro
AU  - Tanaka M
FAU - Kawano, Kouichiro
AU  - Kawano K
FAU - Mochizuki, Kazuo
AU  - Mochizuki K
FAU - Kamura, Toshiharu
AU  - Kamura T
FAU - Itoh, Kyogo
AU  - Itoh K
FAU - Harada, Mamoru
AU  - Harada M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Female
MH  - HLA-A Antigens/immunology
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Immunoglobulin G/analysis
MH  - Immunotherapy/methods
MH  - Monocytes
MH  - Oncogene Proteins, Viral/*physiology
MH  - Papillomaviridae/pathogenicity
MH  - Repressor Proteins/*physiology
MH  - T-Lymphocytes, Cytotoxic
MH  - Tumor Cells, Cultured
MH  - Uterine Cervical Neoplasms/*immunology/*virology
EDAT- 2005/10/08 09:00
MHDA- 2005/12/16 09:00
CRDT- 2005/10/08 09:00
PHST- 2005/10/08 09:00 [pubmed]
PHST- 2005/12/16 09:00 [medline]
PHST- 2005/10/08 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2005 Nov;27(5):1371-9.