PMID- 16213362
OWN - NLM
STAT- MEDLINE
DCOM- 20051125
LR  - 20231213
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 162
IP  - 2
DP  - 2005 Oct 15
TI  - Detection of ETV6 and RUNX1 gene rearrangements using fluorescence in situ 
      hybridization in Mexican patients with acute lymphoblastic leukemia: experience 
      at a single institution.
PG  - 140-5
AB  - The t(12;21) produces the gene fusion ETV6/RUNX1 and is a frequent rearrangement 
      in childhood ALL, associated with a good prognosis. In Mexico its prevalence has 
      not been reported. This study evaluated a group of consecutive Mexican children 
      with newly diagnosed ALL, to detect the fusion using fluorescence in situ 
      hybridization (FISH). Seventy-one bone marrow samples were analyzed with FISH, 
      using ETV6/RUNX1 DNA probes. Abnormalities of ETV6, RUNX1, or both were found in 
      31 of the 71 (44%) patients. Six showed ETV6/RUNX1 fusion and 17, with extra 
      RUNX1 copies, presented an additional chromosome 21 or dup(21)(q22). Five 
      patients had structural changes in ETV6, and three patients showed extra copies 
      of ETV6 and RUNX1 from polysomy of chromosomes 12 and 21. Our results revealed a 
      fusion in 8.5% of the 71 cases analyzed. This frequency is lower than that 
      observed in other populations (9.5-32%). The structural rearrangements resulting 
      in RUNX1 extra copies were found in 9.8% of patients, which is close to the range 
      reported (1.5-9.7%) by other authors. Due to the prevalence of RUNX1 
      overrepresentation in our population and its unknown prognostic significance, 
      further studies should be conducted in consecutive children with ALL, to 
      correlate this abnormality with the patients' follow-up.
FAU - Perez-Vera, Patricia
AU  - Perez-Vera P
AD  - Department of Research in Human Genetics, Instituto Nacional de Pediatria 
      Insurgentes Sur 3700-C, Col. Insurgentes Cuicuilco, Mexico D.F. 04530, Mexico.
FAU - Montero-Ruiz, Oreth
AU  - Montero-Ruiz O
FAU - Frias, Sara
AU  - Frias S
FAU - Ulloa-Aviles, Veronica
AU  - Ulloa-Aviles V
FAU - Cardenas-Cardos, Rocio
AU  - Cardenas-Cardos R
FAU - Paredes-Aguilera, Rogelio
AU  - Paredes-Aguilera R
FAU - Rivera-Luna, Roberto
AU  - Rivera-Luna R
FAU - Carnevale, Alessandra
AU  - Carnevale A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Dosage
MH  - Gene Fusion
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mexico
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Proto-Oncogene Proteins c-ets/*genetics
MH  - Repressor Proteins/*genetics
MH  - Translocation, Genetic
MH  - ETS Translocation Variant 6 Protein
EDAT- 2005/10/11 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/11 09:00
PHST- 2005/02/28 00:00 [received]
PHST- 2005/03/07 00:00 [revised]
PHST- 2005/03/30 00:00 [accepted]
PHST- 2005/10/11 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/11 09:00 [entrez]
AID - S0165-4608(05)00209-8 [pii]
AID - 10.1016/j.cancergencyto.2005.03.018 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Oct 15;162(2):140-5. doi: 
      10.1016/j.cancergencyto.2005.03.018.