PMID- 16213701
OWN - NLM
STAT- MEDLINE
DCOM- 20061030
LR  - 20151119
IS  - 0954-6111 (Print)
IS  - 0954-6111 (Linking)
VI  - 100
IP  - 4
DP  - 2006 Apr
TI  - Exhaled breath condensate levels of 8-isoprostane, growth related oncogene alpha 
      and monocyte chemoattractant protein-1 in patients with chronic obstructive 
      pulmonary disease.
PG  - 630-8
AB  - Chronic obstructive pulmonary disease (COPD) patients have increased neutrophils 
      and macrophages in their lungs, and inflammation of the airway is related to 
      oxidative stress. This study assessed the levels of 8-isoprostane (an oxidative 
      stress marker) and chemokines related to neutrophil and monocyte inflammation 
      (growth-related oncogene alpha [GROalpha] and monocyte chemoattractant protein-1 
      [MCP-1]) in the airway of ex-smoking COPD patients by exhaled breath condensate 
      (EBC) collection. Thirty-two (28 males) stable COPD patients (14 with FEV(1) 50% 
      [Group 1], 18 with FEV(1) <50% predicted [Group 2]) and 18 non-smoking age and 
      sex-matched controls were studied in this cross-sectional study. EBC was 
      collected using the EcoScreen (Jaeger, Germany) during 10 min of tidal breathing 
      with the nose clipped. Concentrations of 8-isoprostane, GROalpha and MCP-1 were 
      measured by enzyme immunoassays. COPD patients had a higher concentration of 
      8-isoprostane than controls (COPD versus control, P<0.001; Group 1 versus Group 
      2, P=0.045). 8-isoprostane increased across the groups from normal, Group 1 to 
      Group 2 (r=0.64, P<0.001). The median intraquartile range (IQR) levels in pg/ml 
      for GROalpha were 45.3(44.5-46.5), 45.4(44.5-46.0), 46.0(45.6-47.3), whereas 
      MCP-1 levels were 5.3(5.2-5.9), 6.2(5.4-6.9) and 5.7(5.5-6.4) in Group 1, Group 2 
      COPD and control subjects, respectively. GROalpha level was lower in COPD 
      patients when compared to controls (P=0.01). MCP-1 level did not differ between 
      COPD and the control group. 8-isoprostane level, but not GROalpha and MCP-1, in 
      EBC was increased in COPD patients with poorer lung function. This suggests an 
      increased oxidative stress in the airway in patients with more severe COPD.
FAU - Ko, Fanny W S
AU  - Ko FW
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Prince of Wales Hospital, Shatin, New Territories, Hong Kong. fannyko@cuhk.edu.hk
FAU - Lau, Christine Y K
AU  - Lau CY
FAU - Leung, Ting F
AU  - Leung TF
FAU - Wong, Gary W K
AU  - Wong GW
FAU - Lam, Christopher W K
AU  - Lam CW
FAU - Hui, David S C
AU  - Hui DS
LA  - eng
PT  - Journal Article
DEP - 20051006
PL  - England
TA  - Respir Med
JT  - Respiratory medicine
JID - 8908438
RN  - 0 (8-isoprostaglandin A2)
RN  - 0 (Biomarkers)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Prostaglandins A)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers/analysis
MH  - *Breath Tests/methods
MH  - Chemokine CCL2/*analysis
MH  - Chemokine CXCL1
MH  - Chemokines, CXC/*analysis
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*analysis
MH  - Male
MH  - Middle Aged
MH  - Oncogenes
MH  - Oxidative Stress/*physiology
MH  - Prospective Studies
MH  - Prostaglandins A/*analysis
MH  - Pulmonary Disease, Chronic Obstructive/*diagnosis/physiopathology
EDAT- 2005/10/11 09:00
MHDA- 2006/10/31 09:00
CRDT- 2005/10/11 09:00
PHST- 2005/06/10 00:00 [received]
PHST- 2005/08/05 00:00 [accepted]
PHST- 2005/10/11 09:00 [pubmed]
PHST- 2006/10/31 09:00 [medline]
PHST- 2005/10/11 09:00 [entrez]
AID - S0954-6111(05)00332-X [pii]
AID - 10.1016/j.rmed.2005.08.009 [doi]
PST - ppublish
SO  - Respir Med. 2006 Apr;100(4):630-8. doi: 10.1016/j.rmed.2005.08.009. Epub 2005 Oct 
      6.