PMID- 16215732
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20220317
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 57
IP  - 10
DP  - 2005 Nov
TI  - High-throughput DNA typing of HLA-A, -B, -C, and -DRB1 loci by a PCR-SSOP-Luminex 
      method in the Japanese population.
PG  - 717-29
AB  - We have developed a new high-throughput, high-resolution genotyping method for 
      the detection of alleles at the human leukocyte antigen (HLA)-A, -B, -C, and 
      -DRB1 loci by combining polymerase chain reaction (PCR) and sequence-specific 
      oligonucleotide probes (SSOPs) protocols with the Luminex 100 xMAP flow cytometry 
      dual-laser system to quantitate fluorescently labeled oligonucleotides attached 
      to color-coded microbeads. In order to detect the HLA alleles with a frequency of 
      more than 0.1% in the Japanese population, we created 48 oligonucleotide probes 
      for the HLA-A locus, 61 for HLA-B, 34 for HLA-C, and 51 for HLA-DRB1. The 
      accuracy of the PCR-SSOP-Luminex method was determined by comparing it to the 
      nucleotide sequencing method after subcloning into the plasmid vector using 150 
      multinational control samples obtained from the International HLA DNA Exchange 
      University of California Los Angeles. In addition, we performed the 
      PCR-SSOP-Luminex method for HLA allele typing on DNA samples collected from 1,018 
      Japanese volunteers. Overall, the genotyping method exhibited an accuracy of 
      85.91% for HLA-A, 85.03% for HLA-B, 97.32% for HLA-C, and 90.67% for HLA-DRB1 
      using 150 control samples, and 100% for HLA-A and -C, 99.90% for HLA-B, and 
      99.95% for HLA-DRB1 in 1,018 Japanese samples. The PCR-SSOP-Luminex method 
      provides a simple, accurate, and rapid approach toward multiplex genotyping of 
      HLA alleles to the four-digit or higher level of resolution in the Japanese 
      population. It takes only approximately 5 h from DNA extraction to the definition 
      of HLA four-digit alleles at the HLA-A, HLA-B, HLA-C, and HLA-DRB1 loci for 96 
      samples when handled by a single typist.
FAU - Itoh, Yoshiki
AU  - Itoh Y
AD  - Department of Ophthalmology and Visual Science, Yokohama City University Graduate 
      School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. 
      yoshi66@med.yokohama-cu.ac.jp
FAU - Mizuki, Nobuhisa
AU  - Mizuki N
FAU - Shimada, Tsuyako
AU  - Shimada T
FAU - Azuma, Fumihiro
AU  - Azuma F
FAU - Itakura, Mitsuo
AU  - Itakura M
FAU - Kashiwase, Koichi
AU  - Kashiwase K
FAU - Kikkawa, Eri
AU  - Kikkawa E
FAU - Kulski, Jerzy K
AU  - Kulski JK
FAU - Satake, Masahiro
AU  - Satake M
FAU - Inoko, Hidetoshi
AU  - Inoko H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051108
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (DNA Primers)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - DNA Primers
MH  - Gene Frequency
MH  - Genotype
MH  - HLA-A Antigens/*genetics
MH  - HLA-B Antigens/*genetics
MH  - HLA-C Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Japan
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
EDAT- 2005/10/11 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/10/11 09:00
PHST- 2005/06/20 00:00 [received]
PHST- 2005/09/02 00:00 [accepted]
PHST- 2005/10/11 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/10/11 09:00 [entrez]
AID - 10.1007/s00251-005-0048-3 [doi]
PST - ppublish
SO  - Immunogenetics. 2005 Nov;57(10):717-29. doi: 10.1007/s00251-005-0048-3. Epub 2005 
      Nov 8.