PMID- 16216675
OWN - NLM
STAT- MEDLINE
DCOM- 20060117
LR  - 20111117
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 66
IP  - 8
DP  - 2005 Aug
TI  - HLA-DQA1 introns 2 and 3 sequencing: DQA1 sequencing-based typing and 
      characterization of a highly polymorphic microsatellite at intron 3 of DQA1*0505.
PG  - 903-11
AB  - DQA1 class II gene encodes the alpha-chain of the human leukocyte antigen 
      (HLA)-DQ heterodimer. Sequencing-based typing (SBT) for HLA genes is the most 
      powerful methodology described. However, most of the SBT procedures reported for 
      HLA class II genes are not able to define complete exon 2 region. For that 
      purpose, we have characterized introns 2 and 3 from most DQA1 alleles to design 
      amplification procedures that were able to obtain complete exon 2 and 3 sequences 
      from DQA1 genes. This coding information allowed us to reduce the number of 
      ambiguities for DQA1 typing. DQA1 intron 2 and 3 characterization demonstrated 
      the presence of two polymorphisms for alleles with the same exons 2 and 3 
      sequence from DQA1*05 group. Different samples including the DQA1*050101 alleles 
      showed a single nucleotide polymorphism at position 53 of intron 2 (G53T). 
      Additional haplotypic analysis showed the possible association of T53 allele with 
      the Ax-Cw5-B18-DR17-DQ2 extended haplotype. On the other hand, DQA1*0505 
      sequencing from different control samples noticed the existence of a 
      microsatellite (TTTC/AAAG)n located at position 126 of intron 3. Fragment length 
      analysis demonstrated a high polymorphism for this short tandem repeat system 
      (0505STR), defining alleles that ranged from 8 to 20 repetitions in our 
      population.
FAU - Balas, Antonio
AU  - Balas A
AD  - Histocompatibility and Molecular Biology Department. Regional Transfusion Centre 
      of Madrid, Madrid, Spain.
FAU - Aviles, Maria J
AU  - Aviles MJ
FAU - Alonso-Nieto, Manuela
AU  - Alonso-Nieto M
FAU - Zarapuz, Loreto
AU  - Zarapuz L
FAU - Blanco, Lydia
AU  - Blanco L
FAU - Garcia-Sanchez, Felix
AU  - Garcia-Sanchez F
FAU - Vicario, Jose L
AU  - Vicario JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050725
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DQ alpha-Chains)
RN  - 0 (HLA-DQA1 antigen)
SB  - IM
MH  - Base Sequence
MH  - Exons
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DQ alpha-Chains
MH  - Humans
MH  - Introns/*genetics
MH  - *Microsatellite Repeats
MH  - Molecular Sequence Data
MH  - *Polymorphism, Genetic
MH  - Sequence Analysis, DNA
EDAT- 2005/10/12 09:00
MHDA- 2006/01/18 09:00
CRDT- 2005/10/12 09:00
PHST- 2005/03/03 00:00 [received]
PHST- 2005/10/12 09:00 [pubmed]
PHST- 2006/01/18 09:00 [medline]
PHST- 2005/10/12 09:00 [entrez]
AID - S0198-8859(05)00117-5 [pii]
AID - 10.1016/j.humimm.2005.06.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2005 Aug;66(8):903-11. doi: 10.1016/j.humimm.2005.06.003. Epub 2005 
      Jul 25.