PMID- 16217858
OWN - NLM
STAT- MEDLINE
DCOM- 20060328
LR  - 20201209
IS  - 0260-437X (Print)
IS  - 0260-437X (Linking)
VI  - 26
IP  - 1
DP  - 2006 Jan-Feb
TI  - Advantage of using CBA/N strain mice in a non-radioisotopic modification of the 
      local lymph node assay.
PG  - 5-9
AB  - The murine local lymph node assay (LLNA) is currently recognized as a stand-alone 
      test method for determining the skin sensitizing potential of chemicals. It has 
      been incorporated into the official test guidelines published by some 
      authorities, including the OECD. To avoid the use of radioisotopes, efforts have 
      been made recently to develop non-radioisotopic modifications of the LLNA. A 
      non-radioisotopic modification of the LLNA was developed previously using 
      5-bromo-2'-deoxyuridine (BrdU) incorporation (non-RI LLNA). However, the non-RI 
      LLNA was found to be somewhat less sensitive than the standard assay. This study 
      reports the advantage of using mice of the CBA/N strain in the non-RI LLNA to 
      improve the sensitivity of this method. The non-RI LLNA was performed using 
      CBA/JN and CBA/N mice exposed to one of four confirmed skin sensitizers, 
      2,4-dinitrochlorobenzene (DNCB), eugenol (EG), isoeugenol (IEG) or 
      alpha-hexylcinnamic aldehyde (HCA), and to one non-sensitizer, propylene glycol 
      (PG). The EC3 values for DNCB, IEG, EG, HCA and PG were calculated to be 0.1%, 
      9.6%, 40.6%, 45.5% and >50% in CBA/JN mice and 0.08%, 1.9%, 10.7%, 20.3% and >50% 
      in CBA/N mice, respectively. The EC3 values for DNCB, IEG, EG, HCA and PG in the 
      standard LLNA using CBA/Ca mice and radioisotopes were reported elsewhere as 
      being 0.08%, 1.3%, 13.0%, 8.0% and >50%, respectively. The EC3 values derived 
      from the CBA/N mice in the non-RI LLNA were nearly equivalent to the EC3 values 
      obtained using the standard radioisotopic LLNA with CBA/Ca mice. These data 
      suggest that the use of CBA/N mice may provide a realistic opportunity to develop 
      a version of the LLNA that does not have a requirement for the use of 
      radioisotopes, but which nevertheless has sensitivity approaching, or comparable 
      to, the standard method.
CI  - 2005 John Wiley & Sons, Ltd.
FAU - Takeyoshi, Masahiro
AU  - Takeyoshi M
AD  - Hita Laboratory, Chemicals Evaluation and Research Institute, 3-822, Ishii-machi, 
      Hita-shi, Oita 8770061, Japan. takeyoshi-masahiro@ceri.jp
FAU - Noda, Shuji
AU  - Noda S
FAU - Yamasaki, Kanji
AU  - Yamasaki K
FAU - Kimber, Ian
AU  - Kimber I
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Allergens)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Irritants)
RN  - 101-86-0 (hexyl cinnamic aldehyde)
RN  - 3T8H1794QW (Eugenol)
RN  - 5M0MWY797U (isoeugenol)
RN  - 7864XYD3JJ (Acrolein)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Acrolein/analogs & derivatives/toxicity
MH  - Allergens/*toxicity
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Dermatitis, Allergic Contact
MH  - Dinitrochlorobenzene/toxicity
MH  - Eugenol/analogs & derivatives/toxicity
MH  - Female
MH  - Irritants/toxicity
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/cytology/drug effects/metabolism
MH  - Mice
MH  - *Mice, Inbred CBA
MH  - Predictive Value of Tests
MH  - Species Specificity
EDAT- 2005/10/12 09:00
MHDA- 2006/03/29 09:00
CRDT- 2005/10/12 09:00
PHST- 2005/10/12 09:00 [pubmed]
PHST- 2006/03/29 09:00 [medline]
PHST- 2005/10/12 09:00 [entrez]
AID - 10.1002/jat.1096 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2006 Jan-Feb;26(1):5-9. doi: 10.1002/jat.1096.