PMID- 16219548
OWN - NLM
STAT- MEDLINE
DCOM- 20051202
LR  - 20061115
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 33
IP  - 10
DP  - 2005 Oct
TI  - Enhanced engraftment of umbilical cord blood-derived stem cells in NOD/SCID mice 
      by cotransplantation of a second unrelated cord blood unit.
PG  - 1249-56
AB  - OBJECTIVE: Umbilical cord blood (UCB) is considered as an attractive alternative 
      source of hematopoietic stem cells for allogeneic stem cell transplantations in 
      patients who lack human leukocyte antigen (HLA)-matched donors. However, the low 
      cell dose adversely affects hematopoietic recovery and therefore limits 
      application of UCB transplantation in adults. Transplantation of multiple UCB 
      units could be a strategy to overcome cell dose limitations. MATERIALS AND 
      METHODS: To investigate the effect of double cord transplantation, nonobese 
      diabetic/severe combined immunodeficient mice were transplanted with human 
      hematopoietic progenitor cells (CD34(+)) derived from two UCB units with HLA 
      disparity. Human cell engraftment and donor origin was determined by flow 
      cytometry. RESULTS: Double CB transplantation resulted in increased engraftment 
      levels in the bone marrow and peripheral blood in comparison with recipients of a 
      single unit. Because this effect could be due to the higher cell dose (2.10(5) vs 
      1.10(5) cells), double CB transplantation was compared with single units 
      containing equal cell numbers (2.10(5)). In some cases, engraftment levels in 
      recipients of single units containing 2.10(5) cells were significantly higher 
      than after transplantation of 1.10(5) cells. These engraftment levels were 
      similar to those observed after double CB transplantation. Chimerism analysis 
      indicated that increased engraftment in recipients of two units was predominantly 
      derived from one unit, whereas in other cases the contribution of the two units 
      was similar. CONCLUSION: These results indicate that engraftment may be enhanced 
      by addition of a second unrelated CB that might be attributed to a cell dose 
      effect or due to a graft-facilitating effect.
FAU - Nauta, Alma J
AU  - Nauta AJ
AD  - Laboratory of Experimental Hematology, Department of Hematology, Leiden 
      University Medical Center, Leiden, the Netherlands. nauta@lumc.nl
FAU - Kruisselbrink, Alwine B
AU  - Kruisselbrink AB
FAU - Lurvink, Ellie
AU  - Lurvink E
FAU - Mulder, Arend
AU  - Mulder A
FAU - Claas, Frans H
AU  - Claas FH
FAU - Noort, Willy A
AU  - Noort WA
FAU - Willemze, Roel
AU  - Willemze R
FAU - Fibbe, Willem E
AU  - Fibbe WE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antigens, CD34/metabolism
MH  - *Blood Donors
MH  - *Cord Blood Stem Cell Transplantation/methods
MH  - Fetal Blood/cytology/physiology
MH  - Graft Survival/*physiology
MH  - Hematopoiesis/*physiology
MH  - Hematopoietic Stem Cells/cytology/physiology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Recovery of Function/*physiology
MH  - Transplantation, Heterologous
MH  - Transplantation, Homologous
EDAT- 2005/10/13 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/10/13 09:00
PHST- 2005/04/29 00:00 [received]
PHST- 2005/06/13 00:00 [revised]
PHST- 2005/06/14 00:00 [accepted]
PHST- 2005/10/13 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/10/13 09:00 [entrez]
AID - S0301-472X(05)00299-7 [pii]
AID - 10.1016/j.exphem.2005.06.019 [doi]
PST - ppublish
SO  - Exp Hematol. 2005 Oct;33(10):1249-56. doi: 10.1016/j.exphem.2005.06.019.