PMID- 16219981
OWN - NLM
STAT- MEDLINE
DCOM- 20060505
LR  - 20131121
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 22
IP  - 9
DP  - 2005 Sep
TI  - Interleukin-18 (IL-18) mRNA expression and localization of IL-18 mRNA-expressing 
      cells in the mouse uterus.
PG  - 1003-10
AB  - Interleukin-18 (IL-18) belongs to the interleukin-1 family and was identified as 
      an interferon-gamma inducing factor. We investigated IL-18 mRNA-expressing cells 
      in the mouse uterus. By RNase protection assay, IL-18 mRNA and alpha subunit of 
      IL-18 receptor mRNA were detected in the uterus. In the uterus, IL-18 mRNA levels 
      increased during sexual maturation. In situ hybridization analysis demonstrated 
      IL-18 mRNA-expressing cells in the mouse uterus of different ages. At 21 days of 
      age, IL-18 mRNA-expressing cells were detected in the luminal epithelial cells 
      and stromal cells although the IL-18 mRNA signal was weak. At 42 days of age, 
      IL-18 mRNA signal was mainly detected in the stromal cells located near the 
      myometrium, and in some of the luminal and glandular epithelial cells. In the 
      uterus of 63-day-old adult mice, a strong hybridization signal for IL-18 mRNA was 
      detected at estrus, but was weak at diestrus. IL-18 mRNA was mainly detected in 
      the glandular epithelial cells and stromal cells. The effect of estradiol-17beta 
      (E(2)) on IL-18 mRNA-expressing cells in the uterus was examined in 
      ovariectomized mice. In oil-treated mice IL-18 mRNA signal was localized in 
      luminal epithelial cells and stromal cells, while in E(2)-treated mice IL-18 mRNA 
      signal was localized in stromal cells alone. These results suggest that the mouse 
      uterus has an IL-18 system, and IL-18 exerts a physiological role within the 
      uterus in a paracrine manner, and that IL-18 gene expression is regulated by 
      estrogen.
FAU - Kusumoto, Kenji
AU  - Kusumoto K
AD  - Department of Biology, Faculty of Science, Okayama University, Japan.
FAU - Murakami, Yousuke
AU  - Murakami Y
FAU - Otsuki, Mariko
AU  - Otsuki M
FAU - Kanayama, Munetoshi
AU  - Kanayama M
FAU - Takeuchi, Sakae
AU  - Takeuchi S
FAU - Takahashi, Sumio
AU  - Takahashi S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-18)
RN  - 0 (RNA, Messenger)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - DNA Primers
MH  - Epithelial Cells/drug effects/*metabolism
MH  - Estradiol/pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - In Situ Hybridization
MH  - Interleukin-18/*metabolism
MH  - Mice
MH  - Paracrine Communication/*physiology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Uterus/*metabolism
EDAT- 2005/10/13 09:00
MHDA- 2006/05/06 09:00
CRDT- 2005/10/13 09:00
PHST- 2005/10/13 09:00 [pubmed]
PHST- 2006/05/06 09:00 [medline]
PHST- 2005/10/13 09:00 [entrez]
AID - JST.JSTAGE/zsj/22.1003 [pii]
AID - 10.2108/zsj.22.1003 [doi]
PST - ppublish
SO  - Zoolog Sci. 2005 Sep;22(9):1003-10. doi: 10.2108/zsj.22.1003.