PMID- 16220194
OWN - NLM
STAT- MEDLINE
DCOM- 20131205
LR  - 20181201
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 57
IP  - 5
DP  - 2005 Oct 25
TI  - Migration of intravenously grafted mesenchymal stem cells to injured heart in 
      rats.
PG  - 566-72
AB  - The present study aimed to determine the role of tissue injury in migration of 
      mesenchymal stem cells (MSCs) intravenously transplanted into heart and to 
      establish experimental basis for improving stem cell therapy in its targeting and 
      effectiveness. MSCs were isolated from bone marrow of male Sprague-Dawley rats 
      and purified by density centrifuge and adhered to the culture plate in vitro. 
      Female rats were divided randomly into four groups. Myocardial ischemia (MI) 
      transplanted group received MSCs infusion through tail vein 3 h after MI and 
      compared with sham-operated group or normal group with MSCs infusion, or control 
      group received culture medium infusion. MI was created in female rats by ligating 
      the left anterior descending coronary artery. The heart was harvested 1 week and 
      8 weeks after transplantation. The characteristics of migration of MSCs to heart 
      were detected with expression of sry gene of Y chromosome by using fluorescence 
      in situ hybridization (FISH). Ultrastructural changes of the ischemic myocardium 
      of the recipient rats were observed by transmission electron microscope (TEM). 
      One week or 8 weeks after transplantation, sry positive cells were observed in 
      the cardiac tissue in both of MI transplanted group and sham-operated group, the 
      number of sry positive cells being significantly higher in MI transplanted group 
      (P<0.01). No significant difference was found in the number of sry positive cells 
      between 1 week and 8 weeks after transplantation. No sry positive cells were 
      observed in the hearts of control and normal group. In addition, the 
      ultrastructure of some cells located in the peri-infarct area of MI rats with 
      MSCs transplantation was similar to that of MSCs cultured in vitro. These results 
      indicate that MSCs are capable of migrating towards ischemic myocardium in vivo 
      and the fastigium of migration might appear around 1 week after MI. The tissue 
      injury and its degree play an important role in the migration of MSCs.
FAU - Jiang, Wen-Hui
AU  - Jiang WH
AD  - Cardiovascular Department, the First Hospital of Xi'an Jiaotong University, 
      Ministry of Education, Xi'an 710061, China.
FAU - Ma, Ai-Qun
AU  - Ma AQ
FAU - Zhang, Yan-Min
AU  - Zhang YM
FAU - Han, Ke
AU  - Han K
FAU - Liu, Yu
AU  - Liu Y
FAU - Zhang, Zeng-Tie
AU  - Zhang ZT
FAU - Wang, Ting-Zhong
AU  - Wang TZ
FAU - Huang, Xin
AU  - Huang X
FAU - Zheng, Xiao-Pu
AU  - Zheng XP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Cell Tracking
MH  - Female
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocardial Ischemia/*therapy
MH  - Myocardium/ultrastructure
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/10/13 09:00
MHDA- 2013/12/16 06:00
CRDT- 2005/10/13 09:00
PHST- 2005/10/13 09:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2005/10/13 09:00 [entrez]
PST - ppublish
SO  - Sheng Li Xue Bao. 2005 Oct 25;57(5):566-72.