PMID- 16220347
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20181113
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 57
IP  - 10
DP  - 2005 Nov
TI  - Intrahaplotype and interhaplotype pairing of bovine leukocyte antigen DQA and DQB 
      molecules generate functional DQ molecules important for priming CD4(+) 
      T-lymphocyte responses.
PG  - 750-62
AB  - Antigen-specific CD4(+) T-lymphocyte responses are restricted by major 
      histocompatibility complex class II molecules, which influence T-cell priming 
      during infection. Human leukocyte antigen (HLA) and bovine leukocyte antigen 
      (BoLA) DRB3 and DQ genes are polymorphic, but unlike HLA, many BoLA haplotypes 
      have duplicated DQ genes, and antibody-blocking studies indicated that BoLA-DQ 
      molecules present various pathogen epitopes. Limited experimentation also 
      suggested that BoLA-DQ molecules formed by interhaplotype pairing of A and B 
      chains are functional. To compare antigen presentation by DR and DQ molecules and 
      to definitively demonstrate functional BoLA-DQ molecules derived from 
      interhaplotype pairing, different combinations of DR or DQ A and B proteins were 
      expressed with CD80 in 293-F cells for use as antigen-presenting cells (APCs). 
      This approach identified 11 unique restriction elements including five DR and six 
      DQ pairs for antigen-specific CD4(+) T-cell responses against tick-transmitted 
      bovine hemoparasites Anaplasma marginale or Babesia bovis. Interhaplotype pairing 
      of DQ A and B molecules was demonstrated. Testing of six expressed DQA/B pairs 
      from an animal with duplicated DQ haplotypes (DH16A/DH22H) demonstrated that an 
      interhaplotype pair, DQA*2206/DQB*1301, presented A. marginale peptide B. In 
      DH22H and DH16A homozygous animals, DQA*2206 was tightly linked with DQB*1402, 
      and DQA*22021 was linked with DQB*1301. APCs from these donors could not present 
      peptide B, confirming that DQA*2206/DQB*1301 encoded a functional interhaplotype 
      pair. Functional BoLA-DQ molecules are generated by both intrahaplotype and 
      interhaplotype pairing of A and B chains and play a similar role to BoLA-DR in 
      priming helper T-cell responses to important pathogens.
FAU - Norimine, Junzo
AU  - Norimine J
AD  - Department of Veterinary Microbiology and Pathology, Washington State University, 
      Pullman, WA 99164-7040, USA. nori@vetmed.wsu.edu
FAU - Brown, Wendy C
AU  - Brown WC
LA  - eng
GR  - R01-AI053692/AI/NIAID NIH HHS/United States
GR  - R01-AI30136/AI/NIAID NIH HHS/United States
GR  - R01-AI44005/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20051108
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Antigens, Protozoan)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (BoLA-DQA antigen)
RN  - 0 (BoLA-DQB antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (p44 protein, Anaplasma phagocytophila)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anaplasma marginale/immunology
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Protozoan/immunology
MH  - B7-1 Antigen/biosynthesis/immunology
MH  - B7-2 Antigen/biosynthesis/immunology
MH  - Babesia bovis/immunology
MH  - Bacterial Outer Membrane Proteins/immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology
MH  - Cattle
MH  - Cell Line
MH  - Cell Proliferation
MH  - *Haplotypes
MH  - Histocompatibility Antigens Class II/biosynthesis/genetics/*immunology
MH  - Humans
MH  - Molecular Sequence Data
EDAT- 2005/10/13 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/10/13 09:00
PHST- 2005/05/26 00:00 [received]
PHST- 2005/08/10 00:00 [accepted]
PHST- 2005/10/13 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/10/13 09:00 [entrez]
AID - 10.1007/s00251-005-0045-6 [doi]
PST - ppublish
SO  - Immunogenetics. 2005 Nov;57(10):750-62. doi: 10.1007/s00251-005-0045-6. Epub 2005 
      Nov 8.