PMID- 16220551
OWN - NLM
STAT- MEDLINE
DCOM- 20060612
LR  - 20191210
IS  - 1545-5009 (Print)
IS  - 1545-5009 (Linking)
VI  - 46
IP  - 7
DP  - 2006 Jun
TI  - Rapid and accurate determination of MYCN copy number and 1p deletion in 
      neuroblastoma by quantitative PCR.
PG  - 820-4
AB  - MYCN amplification and 1p36 deletion are adverse prognostic factors in 
      neuroblastoma, and rapid accurate determination of MYCN amplification is 
      essential for risk stratification. MYCN copy number and 1p36 deletion status were 
      determined by fluorescence in situ hybridization (FISH) and real time PCR in a 
      diagnostic pathology laboratory setting on 35 consecutive patients with 
      neuroblastoma. The PCR technique was technically successful in all cases and 
      results were generally available within 24 hr of biopsy. There was no discordance 
      between FISH and PCR results. Real time PCR is a reliable, accurate, and simple 
      technique that can be applied to small neuroblastoma biopsies allowing rapid 
      diagnosis.
CI  - (c) 2005 Wiley-Liss, Inc.
FAU - Anderson, John
AU  - Anderson J
AD  - Department of Oncology, Great Ormond Street Hospital, Unit of Molecular 
      Haematology and Cancer Biology, Institute of Child Health, London, United 
      Kingdom. j.anderson@ich.ucl.ac.uk
FAU - Gibson, Sian
AU  - Gibson S
FAU - Williamson, Dan
AU  - Williamson D
FAU - Rampling, Dyanne
AU  - Rampling D
FAU - Austin, Catherine
AU  - Austin C
FAU - Shipley, Janet
AU  - Shipley J
FAU - Sebire, Neil
AU  - Sebire N
FAU - Brock, Penelope
AU  - Brock P
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pediatr Blood Cancer
JT  - Pediatric blood & cancer
JID - 101186624
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Child, Preschool
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 1
MH  - *Gene Amplification
MH  - *Genes, myc
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Neuroblastoma/genetics/*pathology
MH  - Polymerase Chain Reaction/*methods
MH  - Polymorphism, Single Nucleotide
EDAT- 2005/10/13 09:00
MHDA- 2006/06/13 09:00
CRDT- 2005/10/13 09:00
PHST- 2005/10/13 09:00 [pubmed]
PHST- 2006/06/13 09:00 [medline]
PHST- 2005/10/13 09:00 [entrez]
AID - 10.1002/pbc.20311 [doi]
PST - ppublish
SO  - Pediatr Blood Cancer. 2006 Jun;46(7):820-4. doi: 10.1002/pbc.20311.