PMID- 16221175
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20181113
IS  - 0085-2538 (Print)
IS  - 1523-1755 (Electronic)
IS  - 0085-2538 (Linking)
VI  - 68
IP  - 5
DP  - 2005 Nov
TI  - Kidney tubular epithelium is restored without replacement with bone 
      marrow-derived cells during repair after ischemic injury.
PG  - 1956-61
AB  - The kidney has the ability to restore the structural and functional integrity of 
      the proximal tubule, which undergoes extensive epithelial cell death after 
      prolonged exposure to ischemia. In order to study the role that adult bone 
      marrow-derived stem cells might play in kidney remodeling after injury, we 
      employed a murine model of ischemia/reperfusion (I/R) injury in which the degree 
      of injury, dysfunction, repair, tubular cell proliferation and functional 
      recovery have been characterized [Park KM, et al, J Biol Chem 276:11870-11876, 
      2001]. We generated chimeric mice using marrow from mice expressing the bacterial 
      LacZ gene, or the enhanced green fluorescence protein (eGFP) gene, or from male 
      mice transplanted into female mice. The establishment of chimerism was confirmed 
      at 6 weeks following transplantation in each case. I/R injury was induced in 
      chimeric mice by occluding the renal arteries and veins with microaneurysm clamps 
      for 30 minutes. After functional recovery in the eGFP chimeras, although there 
      were many interstitial cells, no tubular cells were derived from bone marrow 
      cells. In the bacterial beta-galactosidase (beta-gal) chimeric mice we found 
      evidence of mammalian (endogenous) beta-gal by 
      5-bomo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) staining, but not 
      bacterial beta-gal in tubule cells. Detection of the Y chromosome by fluorescence 
      in situ hybridization (FISH) in the postischemic kidneys of gender-mismatched 
      chimeras revealed Y chromosome positivity only in the nuclei of interstitial 
      cells, when scrutinized by deconvolution microscopy. In our model of I/R injury 
      there was a large amount of proliferation of surviving, injured tubular cells 
      indicating that the injured tubule is repopulated by daughter cells of surviving 
      tubular cells. Analysis of the phenotype of interstitial and vascular cells 
      following I/R injury revealed small numbers of peritubular endothelial cells to 
      be derived from bone marrow cells that may serve in the repair process.
FAU - Duffield, Jeremy S
AU  - Duffield JS
AD  - Renal Division, Brigham and Women's Hospital, Department of Medicine, Harvard 
      Medical School, Boston, MA 02115, USA. jduffield@rics.bwh.harvard.edu
FAU - Bonventre, Joseph V
AU  - Bonventre JV
LA  - eng
GR  - R01 DK072381-05/DK/NIDDK NIH HHS/United States
GR  - DK 72381/DK/NIDDK NIH HHS/United States
GR  - R01 DK039773-24/DK/NIDDK NIH HHS/United States
GR  - P01 DK038452-160006/DK/NIDDK NIH HHS/United States
GR  - R37 DK039773/DK/NIDDK NIH HHS/United States
GR  - DK 39773/DK/NIDDK NIH HHS/United States
GR  - R01 DK072381/DK/NIDDK NIH HHS/United States
GR  - DK 38452/DK/NIDDK NIH HHS/United States
GR  - P01 DK038452/DK/NIDDK NIH HHS/United States
GR  - R01 DK039773/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
SB  - IM
CIN - Kidney Int. 2006 Dec;70(11):2040; author reply 2040-1. PMID: 17130826
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell Division
MH  - Epithelial Cells/cytology/physiology
MH  - Humans
MH  - Ischemia/*pathology/*physiopathology
MH  - Kidney Tubules/*cytology/*physiology
PMC - PMC2915580
MID - NIHMS155503
EDAT- 2005/10/14 09:00
MHDA- 2006/01/21 09:00
PMCR- 2010/08/04
CRDT- 2005/10/14 09:00
PHST- 2005/10/14 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/10/14 09:00 [entrez]
PHST- 2010/08/04 00:00 [pmc-release]
AID - S0085-2538(15)51072-7 [pii]
AID - 10.1111/j.1523-1755.2005.00629.x [doi]
PST - ppublish
SO  - Kidney Int. 2005 Nov;68(5):1956-61. doi: 10.1111/j.1523-1755.2005.00629.x.