PMID- 16221241
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 68
IP  - 5
DP  - 2005 Nov
TI  - Apparent successful mesothelial cell transplantation hampered by peritoneal 
      activation.
PG  - 2362-7
AB  - BACKGROUND: Mesothelial cell transplantation has been suggested to improve 
      mesothelial repair after surgery, recurrent peritonitis and peritoneal dialysis. 
      METHODS: In this study we evaluated mesothelial cell transplantation during the 
      resolution phase of experimentally thioglycollate-induced peritonitis in rats. To 
      this end 4 x 10(6) DiO-labeled autologous mesothelial cells were transplanted 1 
      week after peritonitis induction. Peritoneal inflammation and permeability 
      characteristics were evaluated after another week. RESULTS: Mesothelial cell 
      transplantation after peritonitis resulted in incorporation of these cells in the 
      parietal mesothelial lining, leading to an acute transient submesothelial 
      thickening which was not seen in transplanted animals without prior peritonitis 
      induction. Long-term functioning of these repopulated mesothelial cells leaded to 
      peritoneal activation as evidenced by a approximately twofold increase in 
      peritoneal lymphocytes (P < 0.01) and omental mast cell counts (P < 0.05), 
      accompanied by the induction of inflammation markers monocyte chemoattractant 
      protein-1 (MCP-1) (P < 0.01) and hyaluronan (P < 0.01) in the transplanted 
      peritonitis group, but not in rats with peritonitis without mesothelial cell 
      transplantation or in control rats without mesothelial cell transplantation (all 
      four parameters P < 0.01). In addition, trapping of transplanted mesothelial 
      cells in the milky spots of omental tissue and lymphatic stomata of the diaphragm 
      both in control and thioglycollate rats seems to increase microvascular 
      permeability, reflected by apparent increased diffusion rates of small solutes 
      and proteins. CONCLUSION: Altogether, our data underscore the importance of 
      controlling peritoneal (patho)physiology and function in mesothelial 
      transplantation protocols.
FAU - Hekking, Liesbeth H P
AU  - Hekking LH
AD  - Department of Molecular Cell Biology and Immunology, Vrije Universiteit 
      University Medical Center, Amsterdam, The Netherlands.
FAU - Zweers, Machteld M
AU  - Zweers MM
FAU - Keuning, Eelco D
AU  - Keuning ED
FAU - Driesprong, Bas A J
AU  - Driesprong BA
FAU - de Waart, Dirk R
AU  - de Waart DR
FAU - Beelen, Robert H J
AU  - Beelen RH
FAU - van den Born, Jacob
AU  - van den Born J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Thioglycolates)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Epithelial Cells/cytology/*transplantation
MH  - Epithelium
MH  - Lymphocytes/immunology
MH  - Male
MH  - Mast Cells/immunology
MH  - Omentum/cytology/immunology
MH  - Peritoneum/*cytology/*immunology
MH  - Peritonitis/chemically induced/immunology/*therapy
MH  - Rats
MH  - Rats, Wistar
MH  - Thioglycolates
EDAT- 2005/10/14 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/10/14 09:00
PHST- 2005/10/14 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/10/14 09:00 [entrez]
AID - S0085-2538(15)51135-6 [pii]
AID - 10.1111/j.1523-1755.2005.00698.x [doi]
PST - ppublish
SO  - Kidney Int. 2005 Nov;68(5):2362-7. doi: 10.1111/j.1523-1755.2005.00698.x.