PMID- 16224272
OWN - NLM
STAT- MEDLINE
DCOM- 20060428
LR  - 20191210
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 28
IP  - 6
DP  - 2005 Nov-Dec
TI  - Generating renal cancer-reactive T cells using dendritic cells (DCs) to present 
      autologous tumor.
PG  - 551-9
AB  - Dendritic cells (DCs) have been used as professional antigen-presenting cells in 
      vitro to prime T-cell responses. In this study, we generated both CD8+ and CD4+ 
      renal cell carcinoma (RCC)-reactive T cells using a completely autologous system 
      of DCs presenting engulfed whole-tumor cells. We compared DCs presenting RCC 
      tumor cells in different preparations and found ultraviolet-irradiated apoptotic 
      tumor cells to be more immunogenic than necrotic tumor cells or live untreated 
      tumor cells in generating tumor-reactive T cells. In analyzing the T cells 
      generated in this fashion, a CD8+ RCC-reactive T-cell clone generated from a 
      patient recognized an epitope derived from fibroblast growth factor 5 in the 
      context of human leukocyte antigen (HLA) B44*02. CD4+ T cells generated from 
      another patient recognized multiple allogeneic RCC lines expressing 
      HLA-DRbeta1*04, indicating a common shared tumor antigen presented by 
      HLA-DRbeta1*04. The technique of using DCs to present whole-tumor cells can 
      consistently generate both CD4+ and CD8+ RCC-reactive T cells for use in both 
      antigen identification and therapeutic protocols.
FAU - Wang, Qiong J
AU  - Wang QJ
AD  - Surgery Branch, National Cancer Institute, National Institutes of Health, 
      Bethesda, MD 20892, USA.
FAU - Hanada, Ken-ichi
AU  - Hanada K
FAU - Perry-Lalley, Donna
AU  - Perry-Lalley D
FAU - Bettinotti, Maria P
AU  - Bettinotti MP
FAU - Karpova, Tatiana
AU  - Karpova T
FAU - Khong, Hung T
AU  - Khong HT
FAU - Yang, James C
AU  - Yang JC
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Lipopolysaccharide Receptors)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/*immunology
MH  - Apoptosis
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - COS Cells
MH  - Carcinoma, Renal Cell/*immunology
MH  - Cell Line, Tumor
MH  - Chlorocebus aethiops
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Kidney Neoplasms/*immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Lipopolysaccharide Receptors/immunology
MH  - Necrosis
MH  - Ultraviolet Rays
EDAT- 2005/10/15 09:00
MHDA- 2006/04/29 09:00
CRDT- 2005/10/15 09:00
PHST- 2005/10/15 09:00 [pubmed]
PHST- 2006/04/29 09:00 [medline]
PHST- 2005/10/15 09:00 [entrez]
AID - 00002371-200511000-00005 [pii]
AID - 10.1097/01.cji.0000175495.13476.1f [doi]
PST - ppublish
SO  - J Immunother. 2005 Nov-Dec;28(6):551-9. doi: 10.1097/01.cji.0000175495.13476.1f.