PMID- 16229889
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20131121
IS  - 0147-619X (Print)
IS  - 0147-619X (Linking)
VI  - 55
IP  - 2
DP  - 2006 Mar
TI  - Analysis of the beta-lactamase plasmid of borderline methicillin-susceptible 
      Staphylococcus aureus: focus on bla complex genes and cadmium resistance 
      determinants cadD and cadX.
PG  - 114-27
AB  - Borderline methicillin-susceptible Staphylococcus aureus strains are a rather 
      homogeneous group, characterized by MICs of penicillinase-resistant penicillins 
      (PRPs) at or just below the susceptibility breakpoint. Other features unique to 
      this group include the presence of a pBW15-like beta-lactamase plasmid, the 
      association with phage complex 94/96, and the production of a PRP-hydrolyzing 
      beta-lactamase activity in addition to the classical penicillinase activity. The 
      four HindIII fragments of pBORa53, a pBW15-like plasmid from the well-studied 
      borderline S. aureus strain a53, were cloned in Escherichia coli, sequenced and 
      analyzed. The plasmid (17,334 bp in size) contains 14 open reading frames (ORFs) 
      and a complete copy of transposon Tn552, which harbors the three genes of the bla 
      complex (blaZ, blaR1, and blaI) necessary for penicillinase production. Among the 
      other 11 ORFs identified, two were homologous to cadmium resistance determinants 
      of Staphylococcus lugdunensis and to the cadD and cadX genes recently detected in 
      S. aureus. Consistent with this, strain a53 was found to be cadmium resistant. 
      From a collection of 30 S. aureus isolates with borderline PRP MIC levels, 27 
      matched strain a53 in the positive amplification reactions with all of the four 
      primer pairs targeting the cadD-cadX region, the presence of the 17.3-kb plasmid, 
      and the level of cadmium resistance. The well-established S. aureus laboratory 
      strain ATCC 29213 was also found to express cadD-cadX-mediated cadmium 
      resistance. pBORa53 could be re-isolated from transformants obtained by 
      transferring it into a PRP-susceptible recipient. However, while the 
      transformants demonstrated levels of cadmium and penicillin resistance similar to 
      those of strain a53, they remained fully susceptible to PRPs.
FAU - Massidda, Orietta
AU  - Massidda O
AD  - Department of Biomedical Sciences and Technologies, Section of Medical 
      Microbiology, University of Cagliari Medical School, Via Porcell 4, 09100 
      Cagliari, Italy. omassid@caxca1.unica.it
FAU - Mingoia, Marina
AU  - Mingoia M
FAU - Fadda, Daniela
AU  - Fadda D
FAU - Whalen, Michael B
AU  - Whalen MB
FAU - Montanari, Maria Pia
AU  - Montanari MP
FAU - Varaldo, Pietro E
AU  - Varaldo PE
LA  - eng
SI  - GENBANK/AY917098
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051017
PL  - United States
TA  - Plasmid
JT  - Plasmid
JID - 7802221
RN  - 0 (Bacterial Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (cadD protein, Staphylococcus aureus)
RN  - 00BH33GNGH (Cadmium)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Bacterial Proteins/chemistry/*genetics
MH  - Base Sequence
MH  - Cadmium/*physiology
MH  - Cloning, Molecular
MH  - Drug Resistance, Bacterial/genetics
MH  - Membrane Proteins/*genetics
MH  - Methicillin Resistance/*genetics
MH  - Molecular Sequence Data
MH  - Plasmids/*genetics
MH  - Staphylococcus aureus/drug effects/enzymology/*genetics
MH  - Transformation, Bacterial
MH  - beta-Lactamases/chemistry/*genetics
EDAT- 2005/10/19 09:00
MHDA- 2007/08/24 09:00
CRDT- 2005/10/19 09:00
PHST- 2005/06/01 00:00 [received]
PHST- 2005/07/27 00:00 [revised]
PHST- 2005/08/08 00:00 [accepted]
PHST- 2005/10/19 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2005/10/19 09:00 [entrez]
AID - S0147-619X(05)00072-7 [pii]
AID - 10.1016/j.plasmid.2005.08.001 [doi]
PST - ppublish
SO  - Plasmid. 2006 Mar;55(2):114-27. doi: 10.1016/j.plasmid.2005.08.001. Epub 2005 Oct 
      17.