PMID- 16229915
OWN - NLM
STAT- MEDLINE
DCOM- 20060712
LR  - 20191210
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 65
IP  - 3
DP  - 2006 Jun
TI  - Simultaneous enumeration of viable Enterobacteriaceae and Pseudomonas spp. within 
      three hours by multicolor fluorescence in situ hybridization with vital staining.
PG  - 623-7
AB  - A new means of rapidly and simultaneously counting viable phylogenetically 
      different bacteria was developed. The cyanine dimer dye, BOBO-3 that selectively 
      stains bacteria with damaged membranes were used to evaluate bacterial viability 
      based on membrane integrity. Viable Enterobacteriaceae and Pseudomonas spp. could 
      be selectively detected within three hours using multicolor fluorescence in situ 
      hybridization (FISH) following BOBO-3 staining (BOBO3-FISH).
FAU - Kitaguchi, Akiko
AU  - Kitaguchi A
AD  - Environmental Science and Microbiology, Graduate School of Pharmaceutical 
      Sciences, Osaka University, Suita, Japan.
FAU - Yamaguchi, Nobuyasu
AU  - Yamaguchi N
FAU - Nasu, Masao
AU  - Nasu M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20051014
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (BOBO 3)
RN  - 0 (Carbocyanines)
SB  - IM
MH  - Bacteriological Techniques
MH  - Carbocyanines/*metabolism
MH  - *Colony Count, Microbial
MH  - Enterobacteriaceae/*growth & development/isolation & purification
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Pseudomonas/*growth & development/isolation & purification
MH  - Staining and Labeling/*methods
EDAT- 2005/10/19 09:00
MHDA- 2006/07/13 09:00
CRDT- 2005/10/19 09:00
PHST- 2005/03/25 00:00 [received]
PHST- 2005/08/24 00:00 [revised]
PHST- 2005/09/13 00:00 [accepted]
PHST- 2005/10/19 09:00 [pubmed]
PHST- 2006/07/13 09:00 [medline]
PHST- 2005/10/19 09:00 [entrez]
AID - S0167-7012(05)00288-5 [pii]
AID - 10.1016/j.mimet.2005.09.012 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2006 Jun;65(3):623-7. doi: 10.1016/j.mimet.2005.09.012. Epub 
      2005 Oct 14.